Molecular Biology Reports

, Volume 40, Issue 11, pp 6303–6308

MMP-2, TNF-α and NLRP1 polymorphisms in Chinese patients with ankylosing spondylitis and rheumatoid arthritis

Article

DOI: 10.1007/s11033-013-2743-8

Cite this article as:
Sun, R., Huang, Y., Zhang, H. et al. Mol Biol Rep (2013) 40: 6303. doi:10.1007/s11033-013-2743-8

Abstract

Rheumatoid arthritis (RA) and ankylosing spondylitis (AS) are autoimmune, inflammatory diseases with substantial genetic contributions. Matrix metalloproteinase (MMP)-2, tumor necrosis factor (TNF)-α and NLR family pyrin domain-containing 1 (NLRP1) play important roles in the immune response. We studied the MMP-2 rs243865 C/T, TNF-α rs1800629 A/G, NLRP1 rs878329 C/G and NLRP1 rs6502867 C/T polymorphisms in a Chinese cohort of 520 patients with RA, 100 with AS and 520 controls. Genotyping was performed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Using the MMP-2 rs243865 CC homozygote genotype as the reference group, the CT and TT/CT genotypes were associated with significantly reduced risks of AS. However, logistic regression analyses revealed that the MMP-2 rs243865 C/T polymorphism was not associated with risk of RA. TNF-α rs1800629 A/G, NLRP1 rs878329 C/G and NLRP1 rs6502867 C/T polymorphisms were not associated with risk of RA or AS. These findings suggest that the MMP-2 rs243865 C/T polymorphism is associated with AS development.

Keywords

MMP-2PolymorphismAnkylosing spondylitisRheumatoid arthritisMolecular epidemiology

Abbreviations

CI

Confidence interval

LD

Linkage disequilibrium

MMP-2

Matrix metalloproteinase-2

NLRP1

NLR family pyrin domain-containing 1

OR

Odds ratio

SNP

Single nucleotide polymorphism

TNF-α

Tumor necrosis factor-α

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Rongbin Sun
    • 1
  • Yong Huang
    • 1
  • Hui Zhang
    • 1
  • Ruiping Liu
    • 1
    • 2
  1. 1.Department of Orthopedic TraumaAffiliated Hospital of Nanjing Medical University, Changzhou Second People’s HospitalChangzhouChina
  2. 2.Central LaboratoryAffiliated Hospital of Nanjing Medical University, Changzhou Second People’s HospitalChangzhouChina