Molecular Biology Reports

, Volume 40, Issue 11, pp 6091–6096

Adenovirus conducted connective tissue growth factor on extracellular matrix in trabecular meshwork and its role on aqueous humor outflow facility

  • Ying Su
  • Jingli Cheng
  • Hongtao Liu
  • Feng Wang
  • Shiguang Zhao
Article

DOI: 10.1007/s11033-013-2720-2

Cite this article as:
Su, Y., Cheng, J., Liu, H. et al. Mol Biol Rep (2013) 40: 6091. doi:10.1007/s11033-013-2720-2

Abstract

Deposition of extracellular matrix (ECM) in trabecular meshwork, such as fibronectin, collagen IV, elastin. leads to increased resistance of trabecular meshwork in primary open angle glaucoma (POAG). Connective tissue growth factor (CTGF) is known to regulate the ECM deposits. In this study, we detect the effect of adenovirus conducted CTGF (Adv-CTGF) transfection on either the expression of ECM components or aqueous humor outflow facility. Adv-CTGF was used to transfect rat trabecular meshwork cells in vivo and in vitro. Aqueous humor outflow facility was test by microbeads perfusion. Protein expression of CTGF, fibronectin, and collagen IV was determined using Western blot. In the Adv-CTGF group, the outflow facility displayed a significant decrease from baseline. It appears as though the transfection with Adv-CTGF significantly affects the aqueous humor outflow pattern. A negative correlation between IOP and PEFL indicated that a decrease in the area of bead deposition corresponded to an overall decrease of outflow, leading to an elevated IOP. Adv-CTGF can enhance the expression of CTGF, fibronectin and collagen IV. CTGF is the novel target for treatment of POAG. It is necessary to further study to test inhibition of CTGF expression for treatment of POAG.

Keywords

GlaucomaTrabecular meshworkConnective tissue growth factorMicrobeadFacility

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Ying Su
    • 1
    • 2
  • Jingli Cheng
    • 1
  • Hongtao Liu
    • 1
  • Feng Wang
    • 1
  • Shiguang Zhao
    • 3
  1. 1.Department of OphthalmologyFirst Clinical College of Harbin Medical UniversityHarbinChina
  2. 2.Department of Ophthalmology, Department of Anatomy and NeurobiologyBoston University School of MedicineBostonUSA
  3. 3.Department of NeurosurgeryFirst Clinical College of Harbin Medical UniversityHarbinChina