Article

Molecular Biology Reports

, Volume 40, Issue 2, pp 1693-1699

Open Access This content is freely available online to anyone, anywhere at any time.

Single nucleotide polymorphisms of NR3C1 gene and recurrent depressive disorder in population of Poland

  • Elżbieta GałeckaAffiliated withDepartment of Endocrinology and Metabolic Diseases, Medical University of Łódź
  • , Janusz SzemrajAffiliated withDepartment of Medical Biochemistry, Medical University of Łódź
  • , Małgorzata BieńkiewiczAffiliated withDepartment of Quality Control and Radiological Protection, Medical University of Łódź
  • , Ireneusz MajsterekAffiliated withDepartment of Chemistry and Biochemistry, Medical University of Łódź
  • , Karolina Przybyłowska-SygutAffiliated withDepartment of Chemistry and Biochemistry, Medical University of Łódź
  • , Piotr GałeckiAffiliated withDepartment of Adult Psychiatry, Medical University of Łódź
  • , Andrzej LewińskiAffiliated withDepartment of Endocrinology and Metabolic Diseases, Medical University of ŁódźPolish Mother’s Memorial Hospital–Research Institute Email author 

Abstract

Depressive disorder is a disease characterized by disturbances in the hypothalamo–pituitary–adrenal axis. Abnormalities include the increased level of glucocorticoids (GC) and changes in sensitivity to these hormones. The changes are related to glucocorticoid receptors gene (NR3C1) variants. The NR3C1 gene is suggested to be a candidate gene affecting depressive disorder risk and management. The aim of this study was to investigate polymorphisms within the NR3C1 gene and their role in the susceptibility to recurrent depressive disorder (rDD). 181 depressive patients and 149 healthy ethnically matched controls were included in the study. Single nucleotide polymorphisms were assessed using polymerase chain reaction/restriction fragment length polymorphism method. Statistical significance between rDD patients and controls was observed for the allele and genotype frequencies at three loci: BclI, N363S, and ER22/23EK. The presence of C allele, CC, and GC genotype of BclI polymorphism, G allele and GA genotype for N363S and ER22/23EK variants respectively were associated with increased rDD risk. Two haplotypes indicated higher susceptibility for rDD, while haplotype GAG played a protective role with ORdis 0.29 [95 % confidence interval (CI) = 0.13–0.64]. Data generated from this study support the earlier results that genetic variants of the NR3C1 gene are associated with rDD and suggest further consideration on the possible involvement of these variants in etiology of the disease.

Keywords

Depressive disorder Single nucleotide polymorphism Glucocorticoid receptor