Article

Molecular Biology Reports

, Volume 40, Issue 2, pp 1463-1471

Open Access This content is freely available online to anyone, anywhere at any time.

Interfering growth of malignant melanoma with Ang2-siRNA

  • Biao WangAffiliated withDepartment of Plastic Surgery, First Affiliated Hospital of Fujian Medical University Email author 
  • , Zhaoliang LiuAffiliated withDepartment of Plastic Surgery, First Affiliated Hospital of Fujian Medical University
  • , Mingfeng ZhangAffiliated withCollege of Life Sciences, Fujian Normal University
  • , Xiuying SanAffiliated withDepartment of Plastic Surgery, First Affiliated Hospital of Fujian Medical University
  • , Yanding ZhangAffiliated withCollege of Life Sciences, Fujian Normal University
  • , Weiqiang ZhangAffiliated withDepartment of Plastic Surgery, First Affiliated Hospital of Fujian Medical University
  • , Meishui WangAffiliated withDepartment of Plastic Surgery, First Affiliated Hospital of Fujian Medical University

Abstract

To investigate the intervention therapy effect on the growth of malignant melanoma, we have made an observation of expression levels of Ang2 in malignant melanoma cells, which was transduced by small interfering RNA (Ang2-siRNA) of Ang2 in vitro and in vivo. We successfully constructed Ang2-siRNA lent virus, and constructed nude mice model by transplanting malignant melanoma. Ang2-siRNA lent virus inhibited Ang2 mRNA of malignant melanoma in vitro and in vivo, and inhibited malignant melanoma tumor growth at the same time. Ang2-siRNA lent virus can interfere expression levels of Ang2 in malignant melanoma cells, inhibit tumor growth, and silent Ang2 gene expression, which may pave a new way for clinical gene therapy of malignant melanoma.

Keywords

Ang2 Lent virus RNAi Malignant melanoma