Molecular Biology Reports

, Volume 40, Issue 2, pp 1325–1332

Serum microRNA expression profile as a biomarker for the diagnosis of pertussis

Authors

  • Yiyue Ge
    • Institute of Pathogen Microbiology, Jiangsu Provincial Center for Disease Prevention and Control, Key Laboratories of Enteric Pathogenic Microbiology, Ministry of Health
    • Department of Pathogen BiologyNanjing Medical University
  • Kangchen Zhao
    • Institute of Pathogen Microbiology, Jiangsu Provincial Center for Disease Prevention and Control, Key Laboratories of Enteric Pathogenic Microbiology, Ministry of Health
  • Yuhua Qi
    • Institute of Pathogen Microbiology, Jiangsu Provincial Center for Disease Prevention and Control, Key Laboratories of Enteric Pathogenic Microbiology, Ministry of Health
  • Xiaoyan Min
    • Department of GeriatricsThe First Affiliated Hospital, Nanjing Medical University
  • Zhiyang Shi
    • Institute of Pathogen Microbiology, Jiangsu Provincial Center for Disease Prevention and Control, Key Laboratories of Enteric Pathogenic Microbiology, Ministry of Health
  • Xian Qi
    • Institute of Pathogen Microbiology, Jiangsu Provincial Center for Disease Prevention and Control, Key Laboratories of Enteric Pathogenic Microbiology, Ministry of Health
  • Yunfeng Shan
    • Institute of Pathogen Microbiology, Jiangsu Provincial Center for Disease Prevention and Control, Key Laboratories of Enteric Pathogenic Microbiology, Ministry of Health
  • Lan Cui
    • Institute of Pathogen Microbiology, Jiangsu Provincial Center for Disease Prevention and Control, Key Laboratories of Enteric Pathogenic Microbiology, Ministry of Health
  • Minghao Zhou
    • Institute of Pathogen Microbiology, Jiangsu Provincial Center for Disease Prevention and Control, Key Laboratories of Enteric Pathogenic Microbiology, Ministry of Health
  • Yong Wang
    • Department of Pathogen BiologyNanjing Medical University
  • Hua Wang
    • Institute of Pathogen Microbiology, Jiangsu Provincial Center for Disease Prevention and Control, Key Laboratories of Enteric Pathogenic Microbiology, Ministry of Health
    • Institute of Pathogen Microbiology, Jiangsu Provincial Center for Disease Prevention and Control, Key Laboratories of Enteric Pathogenic Microbiology, Ministry of Health
Article

DOI: 10.1007/s11033-012-2176-9

Cite this article as:
Ge, Y., Zhao, K., Qi, Y. et al. Mol Biol Rep (2013) 40: 1325. doi:10.1007/s11033-012-2176-9

Abstract

Pertussis is a highly contagious, respiratory disease associated with substantial morbidity and mortality. A rapid and reliable diagnostic method is essential for appropriate treatment and prevention. Expression profiles of circulating microRNAs (miRNAs) have been proven as new non-invasive biomarkers for infectious diseases. We aimed to investigated the serum miRNA profile in pertussis patients and explored its potential as a novel diagnostic biomarker for pertussis. Among 664 different miRNAs analyzed using a miRNA array, 50 were overexpressed and 81 were underexpressed in the serum of pertussis patients. Expression levels of seven candidate miRNAs were further evaluated by real-time qRT-PCR. A panel of five miRNAs (miR-202, miR-342-5p, miR-206, miR-487b, miR-576-5p) was confirmed overexpressed in pertussis patients (p < 0.05). Risk score and receiver-operating characteristic (ROC) analysis showed that the area under the curve of the five-member miRNA profile was 0.980. At an optimal cutoff value (0.707), this panel of miRNAs yielded a sensitivity of 97.4 % and a specificity of 94.3 %. These data suggest that the five-member serum miRNA profile may serve as a new biomarker for pertussis diagnosis with high specificity and sensitivity.

Keywords

Serum microRNAProfilePertussisBiomarkerDiagnosis

Supplementary material

11033_2012_2176_MOESM1_ESM.doc (74 kb)
Supplementary material 1 (DOC 74 kb)
11033_2012_2176_MOESM2_ESM.doc (103 kb)
Supplementary material 2 (DOC 103 kb)

Copyright information

© Springer Science+Business Media Dordrecht 2012