Molecular Biology Reports

, Volume 40, Issue 1, pp 477–485

Luteolin and fisetin inhibit the effects of lipopolysaccharide obtained from Porphyromonas gingivalis in human gingival fibroblasts

  • Gloria Gutiérrez-Venegas
  • Anabel Contreras-Sánchez

DOI: 10.1007/s11033-012-2083-0

Cite this article as:
Gutiérrez-Venegas, G. & Contreras-Sánchez, A. Mol Biol Rep (2013) 40: 477. doi:10.1007/s11033-012-2083-0


Periodontitis is an inflammatory process of infectious origin that affects the gums and, in severe cases, destroys connective tissue, leading to loss of the dental organ. Gram-negative Porphyromonas gingivalis bacteria are recovered from patients with chronic periodontitis. The polysaccharide obtained from these bacteria induces the expression of interleukin (IL)-1 beta, tumor necrosis factor, and IL-6. Flavonoids are molecules that participate in the control of inflammatory processes. We studied the role of the flavonoids fisetin, luteolin, myricetin, and morin in inhibiting the activation of mitogen-activated protein kinase (MAPK) and AKT as well as their role in lipopolysaccharide (LPS)-induced cyclooxygenase-2 (COX-2) transcription. All four of these flavonoids were found to inhibit MAPK and AKT. Fisetin and luteolin blocked the activation of MAPK and AKT to levels below basal levels. All of these flavonoids also blocked LPS-mediated COX-2 expression.


Human gingival fibroblastsMitogen-activated protein kinaseLipopolysaccharidePorphyromonas gingivalis

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Gloria Gutiérrez-Venegas
    • 1
  • Anabel Contreras-Sánchez
    • 1
  1. 1.Laboratorio de Bioquímica de la División de Estudios de Posgrado e Investigación, Facultad de OdontologíaUniversidad Nacional Autónoma de MéxicoMéxicoMexico