Molecular Biology Reports

, Volume 40, Issue 1, pp 269–279

Antioxidative, anti-inflammatory, and matrix metalloproteinase inhibitory activities of 20(S)-ginsenoside Rg3 in cultured mammalian cell lines

  • Yu-Mi Shin
  • Hyun-Joo Jung
  • Woo-Yong Choi
  • Chang-Jin Lim
Article

DOI: 10.1007/s11033-012-2058-1

Cite this article as:
Shin, YM., Jung, HJ., Choi, WY. et al. Mol Biol Rep (2013) 40: 269. doi:10.1007/s11033-012-2058-1

Abstract

Ginsenoside Rg3 is one of ginsenosides that are the well-known bioactive principles of Panax ginseng. Among the two stereoisomeric forms of Rg3, 20(S)-ginsenoside Rg3 [20(S)-Rg3] is predominant. 20(S)-Rg3 is capable of suppressing the nitric oxide (NO), reactive oxygen species (ROS) and prostaglandin E2 (PGE2) productions induced by lipopolysaccharide (LPS) in RAW264.7 macrophage cells in a concentration-dependent manner. In the same stimulated macrophages, 20(S)-Rg3 was able to suppress matrix metalloproteinase-9 (MMP-9) activity and suppress cyclooxygenase-2 (COX-2) expression. It suppressed the production of some proinflammatory cytokines, such as TNF-α, IL-1β and IL-6, and the cell mobility enhanced by LPS in the macrophage cells. 20(S)-Rg3 displayed suppressive effect on the ROS level but not on the NO level, and down-regulating effect on MMP-9 but not on MMP-2 in non-stimulated HaCat keratinocytes. 20(S)-Rg3 also exhibited suppressive effect on the MMP-9 gelatinolytic activity enhanced in the HaCat keratinocytes stimulated with tumor necrosis factor-α (TNF-α), one of the major proinflammatory cytokines. However, 20(S)-Rg3 was not able to modulate the NO level even in the presence of TNF-α. Taken together, anti-inflammatory and related antioxidative and MMP-9 inhibitory activities of 20(S)-Rg3, the major stereoisomeric form of ginsenoside Rg3, are confirmed in macrophage and keratinocyte cell lines.

Keywords

20(S)-Ginsenoside Rg3Anti-inflammatoryMatrix metalloproteinaseNitric oxideReactive oxygen species

Abbreviations

20(S)-Rg3

20(S)-Ginsenoside Rg3

COX-2

Cyclooxgenase-2

DCF

Dichlorofluorescein

LPS

Lipopolysaccharide

MMP

Matrix metalloproteinase

MTT

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

NO

Nitric oxide

ROS

Reactive oxygen species

TNF-α

Tumor necrosis factor-α

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Yu-Mi Shin
    • 1
  • Hyun-Joo Jung
    • 2
  • Woo-Yong Choi
    • 1
  • Chang-Jin Lim
    • 1
  1. 1.Department of BiochemistryCollege of Natural Sciences, Kangwon National UniversityChuncheonKorea
  2. 2.Department of AnatomyYonsei University College of MedicineSeoulKorea