Molecular Biology Reports

, Volume 39, Issue 12, pp 11217–11223

Interactions of interleukin-12A and interleukin-12B polymorphisms on the risk of intracranial aneurysm

Authors

  • Li-Juan Li
    • Department of Forensic BiologyWest China School of Preclinical and Forensic Medicine, Sichuan University
  • Xin-Min Pan
    • Department of Forensic PathologyHenan University of Science and Technology
  • Xiutian Sima
    • Department of NeurosurgeryWest China Hospital of Sichuan University
  • Zhao-Hui Li
    • Department of Gastrointestinal and Pancreatic SurgeryLuoyang Central Hospital
  • Lu-Shun Zhang
    • Department of Forensic BiologyWest China School of Preclinical and Forensic Medicine, Sichuan University
  • Hong Sun
    • Laboratory of Molecular and Translational MedicineWest China Institute of Women and Children’s Health, West China Second University Hospital, Sichuan University
  • Yi Zhu
    • Department of Forensic BiologyWest China School of Preclinical and Forensic Medicine, Sichuan University
  • Wei-Bo Liang
    • Department of Forensic BiologyWest China School of Preclinical and Forensic Medicine, Sichuan University
    • Laboratory of Molecular and Translational MedicineWest China Institute of Women and Children’s Health, West China Second University Hospital, Sichuan University
    • Key Laboratory of Obstetric & Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education
    • Department of Forensic BiologyWest China School of Preclinical and Forensic Medicine, Sichuan University
Article

DOI: 10.1007/s11033-012-2031-z

Cite this article as:
Li, L., Pan, X., Sima, X. et al. Mol Biol Rep (2012) 39: 11217. doi:10.1007/s11033-012-2031-z

Abstract

Several lines of evidence indicate that inflammatory processes play pivotal role in the development of intracranial aneurysm (IA). Recently, polymorphisms in the interleukin-12 (IL-12) gene were shown to be associated with immune-mediated inflammatory disease. The aim of this study was to investigate the interactions of IL-12A and IL-12B polymorphisms on the risk of IA in a Chinese population. A total of 422 individuals (including 164 patients with IA and 258 controls) were involved in the study. The polymorphisms (i.e., rs2243115 and rs568408 in IL-12A and rs3212227 in IL-12B) were genotyped by polymerase chain reaction–restriction fragment length polymorphism assay and DNA sequencing. We found an association of the AC/CC genotypes and C allele of IL-12B rs3212227 with an increased risk of IA, compared with the AA genotype and A allele (AC/CC vs. AA: OR = 2.09, 95 % CI: 1.29–3.38; C vs. A: OR = 1.45, 95 % CI: 1.10–1.91). Moreover, a significant gene interaction of IL-12A and IL-12B was evident on the risk of IA, and subjects carrying variant genotypes of IL-12B rs3212227 had an increased risk of IA. In the stratified analysis by gender, the IL-12B rs3212227 AC/CC genotypes had an increased risk of IA compared with the AA genotype in male patients (AC/CC vs. AA: OR = 4.63, 95 % CI: 1.92–11.16). These findings suggest that the IL-12A and IL-12B independently and jointly be involved in the susceptibility to IA.

Keywords

Intracranial aneurysmInterleukin-12AInterleukin-12BInflammationGenetic polymorphism

Copyright information

© Springer Science+Business Media Dordrecht 2012