Molecular Biology Reports

, Volume 39, Issue 7, pp 7513–7523

Characterization of transcriptional activation and inserted-into-gene preference of various transposable elements in the Brassica species

Authors

  • Caihua Gao
    • Chongqing Engineering Research Center for Rapeseed, Center of South Upland Agriculture of Ministry of Education, College of Agronomy and BiotechnologySouthwest University
  • Meili Xiao
    • Chongqing Engineering Research Center for Rapeseed, Center of South Upland Agriculture of Ministry of Education, College of Agronomy and BiotechnologySouthwest University
  • Lingyan Jiang
    • College of Plant Science and TechnologyHuazhong Agricultural University
  • Jiana Li
    • Chongqing Engineering Research Center for Rapeseed, Center of South Upland Agriculture of Ministry of Education, College of Agronomy and BiotechnologySouthwest University
  • Jiaming Yin
    • Chongqing Engineering Research Center for Rapeseed, Center of South Upland Agriculture of Ministry of Education, College of Agronomy and BiotechnologySouthwest University
  • Xiaodong Ren
    • Chongqing Engineering Research Center for Rapeseed, Center of South Upland Agriculture of Ministry of Education, College of Agronomy and BiotechnologySouthwest University
  • Wei Qian
    • Chongqing Engineering Research Center for Rapeseed, Center of South Upland Agriculture of Ministry of Education, College of Agronomy and BiotechnologySouthwest University
  • Ortegón Oscar
    • Chongqing Engineering Research Center for Rapeseed, Center of South Upland Agriculture of Ministry of Education, College of Agronomy and BiotechnologySouthwest University
    • Chongqing Engineering Research Center for Rapeseed, Center of South Upland Agriculture of Ministry of Education, College of Agronomy and BiotechnologySouthwest University
    • Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of EducationJiangxi Agricultural University
    • Chongqing Engineering Research Center for Rapeseed, Center of South Upland Agriculture of Ministry of Education, College of Agronomy and BiotechnologySouthwest University
Article

DOI: 10.1007/s11033-012-1585-0

Cite this article as:
Gao, C., Xiao, M., Jiang, L. et al. Mol Biol Rep (2012) 39: 7513. doi:10.1007/s11033-012-1585-0

Abstract

Transposable elements (TEs) have attracted increasing attention because of their tremendous contributions to genome reorganization and gene variation through dramatic proliferation and excision via transposition. However, less known are the transcriptional activation of various TEs and the characteristics of TE insertion into genomes at the genome-wide level. In the present study, we focused on TE genes for transposition and gene disruption by insertion of TEs in expression sequences of Brassica, to investigate the transcriptional activation of TEs, the biased insertion of TEs into genes, and their salient characteristics. Long terminal repeat (LTR-retrotransposon) accounted for the majority of these active TE genes (70.8%), suggesting that transposition activation varied with TE type. 6.1% genes were interrupted by LTR-retrotransposons, which indicated their preference for insertion into genes. TEs were preferentially inserted into cellular component-specific genes acted as “binding” elements and involved in metabolic processes. TEs have a biased insertion into some host genes that were involved with important molecular functions and TE genes exhibited spatiotemporal expression. These results suggested that various types of transposons differentially contributed to gene variation and affected gene function.

Keywords

Brassica speciesTransposable element (TE) for transpositionGenes disrupted by insertion of TETransposon activation

Supplementary material

11033_2012_1585_MOESM1_ESM.xls (28 kb)
Online Resource 1. Phylogenetic analysis of TE genes for transposition and TEs that inserted in genes. (XLS 28 kb)
11033_2012_1585_MOESM2_ESM.xls (32 kb)
Online Resource 2. Genes disrupted by TE that homologous to the genes known to control traits in Arabidopsis thaliana. (XLS 32 kb)
11033_2012_1585_MOESM3_ESM.xls (546 kb)
Online Resource 3. List of cellular components, biological process and molecular function of the genes that inserted by LTR-retrotranspons, and SINE-retransposons. (XLS 546 kb)

Copyright information

© Springer Science+Business Media B.V. 2012