Molecular Biology Reports

, Volume 39, Issue 5, pp 5875–5881

Co-expression of Oct-4 and Nestin in human breast cancers

  • Caigang Liu
  • Xuezhao Cao
  • Yanjun Zhang
  • Hong Xu
  • Ruishan Zhang
  • Yunfei Wu
  • Ping Lu
  • Feng Jin
Article

DOI: 10.1007/s11033-011-1398-6

Cite this article as:
Liu, C., Cao, X., Zhang, Y. et al. Mol Biol Rep (2012) 39: 5875. doi:10.1007/s11033-011-1398-6

Abstract

The aim is to investigate the clinical implications of the Oct-4 and Nestin protein in human breast cancers. A total of 346 cases including 26 fresh and 320 paraffin-embedded tumor tissues were selected for characterizing the frequency of CD44+CD24 tumor cells by flow cytometry and the differential expression of the stem cell-related genes between CD44+CD24 and non-CD44+CD24 tumor cells was analyzed by PCR Array and immunofluorescence. In comparison with the non-CD44+CD24 tumor cells, the CD44+CD24, particularly for those with high percentage of Oct-4+ and Nestin+, tumor cells had higher tumorigenicity by forming mammospheres in vitro. More importantly, 42 (13.125%) out of 320 tumor tissues were positive for Oct-4 and Nestin staining. Universal analysis and multivariate analysis revealed that the expression of Oct-4 and Nestin was associated significantly with younger age, pathogenic degrees, lymph node metastasis and triple-negative breast cancer independently (P < 0.05) as well as shorter survival (P = 0.001). Oct-4 and Nestin were important regulators of the development of breast cancer, and Oct-4 and Nestin may be used as predictors for the prognosis of breast cancers.

Keywords

Breast cancerStem cellOct-4NestinSurvival

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Caigang Liu
    • 1
  • Xuezhao Cao
    • 2
  • Yanjun Zhang
    • 1
  • Hong Xu
    • 3
  • Ruishan Zhang
    • 3
  • Yunfei Wu
    • 1
  • Ping Lu
    • 1
  • Feng Jin
    • 1
  1. 1.Department of Breast Surgery, General SurgeryThe First Hospital of China Medical UniversityShenyangChina
  2. 2.Department of AnesthesiologyThe First Hospital of China Medical UniversityShenyangChina
  3. 3.Department of Breast SurgeryTumor Hospital of Liaoning ProvinceShenyangChina