Molecular Biology Reports

, Volume 39, Issue 5, pp 5233–5238

PDIA3 mRNA expression and IL-2, IL-4, IL-6, and CRP levels of acute kidney allograft rejection in rat

Article

DOI: 10.1007/s11033-011-1321-1

Cite this article as:
Chen, G., Mi, J., Xiao, M.Z. et al. Mol Biol Rep (2012) 39: 5233. doi:10.1007/s11033-011-1321-1

Abstract

Kidney transplantation to treat end-stage renal disease has evolved rapidly from the first successful transplantations to the current widespread use of grafts from both cadaveric and living donors. But acute rejection is still a strong risk factor for chronic rejection in recipients of renal grafts. To investigate possible mechanisms, we describe a comparison between differentially proteins expression and immune markers profile (IL-2, IL-4, IL-6, and CRP) of acute rejection and the controls. Through quantitative real-time RT-PCR confirmation, PDIA3 mRNA and protein expression levels in serum and transplanted kidney in experiment group was significantly (P < 0.05) higher than that in control group. Immunity analysis showed that plasma IL-2, IL-4, IL-6, and CRP levels were higher in experimental rats than those in control rats. Our data thus indicate that PDIA3 might be potentially involve into the occurence and development of acute rejection response in renal transplantation and increased plasma IL-2, IL-4, IL-6, and CRP levels play an important role to prevent acute kidney allograft rejection in rats.

Keywords

PDIA3 RT-PCR IL-2 IL-4 Acute rejection Renal transplantation 

Abbreviations

IL-2

Interleukin-2

IL-4

Interleukin-4

IL-6

Interleukin-6

CRP

C-reactive protein

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Gang Chen
    • 1
  • Jie Mi
    • 2
  • Ming Zhao Xiao
    • 1
  • Yun Ri Fu
    • 1
  1. 1.Department of UrologyThe First Affiliated Hospital, Chongqing Medical UniversityChongqingChina
  2. 2.Department of EmergencyThe First Affiliated Hospital, Chongqing Medical UniversityChongqingChina