Molecular Biology Reports

, Volume 39, Issue 3, pp 2713–2722

miR-29a and miR-142-3p downregulation and diagnostic implication in human acute myeloid leukemia

Authors

  • Fang Wang
    • National Laboratory of Medical Molecular BiologyInstitute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
  • Xiao-Shuang Wang
    • National Laboratory of Medical Molecular BiologyInstitute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
  • Gui-Hua Yang
    • National Laboratory of Medical Molecular BiologyInstitute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
  • Peng-Fei Zhai
    • National Laboratory of Medical Molecular BiologyInstitute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
  • Zhen Xiao
    • Department of HematologyThe Affiliated Hospital of Inner Mongolia Medical College
  • Liang-Yu Xia
    • Clinical LaboratoryPeking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College
  • Li-Rong Chen
    • The First People’s Hospital of Xinxiang
  • Yu Wang
    • Xinxiang Central Hospital
  • Xiao-Zhong Wang
    • Clinical LaboratoryThe Second Affiliated Hospital to Nanchang University
  • Lai-Xi Bi
    • Department of HematologyThe First Affiliated Hospital of Wenzhou Medical College
  • Nian Liu
    • Department of HematologyThe Military General Hospital of Beijing PLA
  • Yang Yu
    • Department of HematologyBeijing Shijitan Hospital
  • Da Gao
    • Department of HematologyThe Affiliated Hospital of Inner Mongolia Medical College
  • Bin-Tao Huang
    • Department of HematologyThe Affiliated Hospital of Inner Mongolia Medical College
  • Jing Wang
    • Department of HematologyThe Affiliated Hospital of Inner Mongolia Medical College
  • Dao-Bin Zhou
    • Department of HematologyPeking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College
  • Jia-Nan Gong
    • National Laboratory of Medical Molecular BiologyInstitute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
  • Hua-Lu Zhao
    • National Laboratory of Medical Molecular BiologyInstitute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
  • Xiu-Hua Bi
    • National Laboratory of Medical Molecular BiologyInstitute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
  • Jia Yu
    • National Laboratory of Medical Molecular BiologyInstitute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
    • National Laboratory of Medical Molecular BiologyInstitute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
Article

DOI: 10.1007/s11033-011-1026-5

Cite this article as:
Wang, F., Wang, X., Yang, G. et al. Mol Biol Rep (2012) 39: 2713. doi:10.1007/s11033-011-1026-5

Abstract

Expression profiling of microRNAs (miRNAs) in most diseases might be popular and provide the possibility for diagnostic implication, but few studies have accurately quantified the expression level of dysregulated miRNAs in acute myeloid leukemia (AML). In this study, we analyzed the peripheral blood mononuclear cells (PBMCs) from 10 AML patients (subtypes M1 to M5) and six normal controls by miRNA microarray and identified several differentially expressed miRNAs. Among them miR-29a and miR-142-3p were selectively encountered in Northern blot analysis and their significantly decreased expression in AML was further confirmed. Quantitative real-time PCR in 52 primarily diagnosed AML patients and 100 normal controls not only verified the expression properties of these 2 miRNAs, but also established that the expression level of miR-142-3p and miR-29a in PBMCs could be used as novel diagnostic markers. A better diagnostic outcome was achieved by combining miR-29a and miR-142-3p with about 90% sensitivity, 100% specificity, and an area under the ROC curve (AUC) of 0.97. Our results provide insights into the involvement of miRNAs in leukemogenesis, and offer candidates for AML diagnosis and therapeutic strategy.

Keywords

MicroRNAs (miRNAs)Acute myeloid leukemia (AML)Expression profilemiR-29amiR-142-3p

Supplementary material

11033_2011_1026_MOESM1_ESM.doc (98 kb)
Supplementary material 1 (DOC 98 kb)
11033_2011_1026_MOESM2_ESM.doc (181 kb)
Supplementary material 2 (DOC 181 kb)
11033_2011_1026_MOESM3_ESM.doc (376 kb)
Supplementary material 3 (DOC 376 kb)
11033_2011_1026_MOESM4_ESM.doc (110 kb)
Supplementary material 4 (DOC 110 kb)

Copyright information

© Springer Science+Business Media B.V. 2011