Article

Molecular Biology Reports

, Volume 39, Issue 3, pp 2713-2722

First online:

miR-29a and miR-142-3p downregulation and diagnostic implication in human acute myeloid leukemia

  • Fang WangAffiliated withNational Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
  • , Xiao-Shuang WangAffiliated withNational Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
  • , Gui-Hua YangAffiliated withNational Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
  • , Peng-Fei ZhaiAffiliated withNational Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
  • , Zhen XiaoAffiliated withDepartment of Hematology, The Affiliated Hospital of Inner Mongolia Medical College
  • , Liang-Yu XiaAffiliated withClinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College
  • , Li-Rong ChenAffiliated withThe First People’s Hospital of Xinxiang
  • , Yu WangAffiliated withXinxiang Central Hospital
  • , Xiao-Zhong WangAffiliated withClinical Laboratory, The Second Affiliated Hospital to Nanchang University
    • , Lai-Xi BiAffiliated withDepartment of Hematology, The First Affiliated Hospital of Wenzhou Medical College
    • , Nian LiuAffiliated withDepartment of Hematology, The Military General Hospital of Beijing PLA
    • , Yang YuAffiliated withDepartment of Hematology, Beijing Shijitan Hospital
    • , Da GaoAffiliated withDepartment of Hematology, The Affiliated Hospital of Inner Mongolia Medical College
    • , Bin-Tao HuangAffiliated withDepartment of Hematology, The Affiliated Hospital of Inner Mongolia Medical College
    • , Jing WangAffiliated withDepartment of Hematology, The Affiliated Hospital of Inner Mongolia Medical College
    • , Dao-Bin ZhouAffiliated withDepartment of Hematology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College
    • , Jia-Nan GongAffiliated withNational Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
    • , Hua-Lu ZhaoAffiliated withNational Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
    • , Xiu-Hua BiAffiliated withNational Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
    • , Jia YuAffiliated withNational Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College
    • , Jun-Wu ZhangAffiliated withNational Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College Email author 

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Abstract

Expression profiling of microRNAs (miRNAs) in most diseases might be popular and provide the possibility for diagnostic implication, but few studies have accurately quantified the expression level of dysregulated miRNAs in acute myeloid leukemia (AML). In this study, we analyzed the peripheral blood mononuclear cells (PBMCs) from 10 AML patients (subtypes M1 to M5) and six normal controls by miRNA microarray and identified several differentially expressed miRNAs. Among them miR-29a and miR-142-3p were selectively encountered in Northern blot analysis and their significantly decreased expression in AML was further confirmed. Quantitative real-time PCR in 52 primarily diagnosed AML patients and 100 normal controls not only verified the expression properties of these 2 miRNAs, but also established that the expression level of miR-142-3p and miR-29a in PBMCs could be used as novel diagnostic markers. A better diagnostic outcome was achieved by combining miR-29a and miR-142-3p with about 90% sensitivity, 100% specificity, and an area under the ROC curve (AUC) of 0.97. Our results provide insights into the involvement of miRNAs in leukemogenesis, and offer candidates for AML diagnosis and therapeutic strategy.

Keywords

MicroRNAs (miRNAs) Acute myeloid leukemia (AML) Expression profile miR-29a miR-142-3p