Molecular Biology Reports

, Volume 39, Issue 3, pp 2375–2383

DNA barcoding of nymphalid butterflies (Nymphalidae: Lepidoptera) from Western Ghats of India

  • S. S. Gaikwad
  • H. V. Ghate
  • S. S. Ghaskadbi
  • M. S. Patole
  • Y. S. Shouche
Article

DOI: 10.1007/s11033-011-0988-7

Cite this article as:
Gaikwad, S.S., Ghate, H.V., Ghaskadbi, S.S. et al. Mol Biol Rep (2012) 39: 2375. doi:10.1007/s11033-011-0988-7

Abstract

We have checked the utility of DNA barcoding for species identification of nymphalid butterflies from Western Ghats of India by using 650 bp sequence of mitochondrial gene cytochrome c oxidase subunit I. Distinct DNA barcoding gap (i.e. difference between intraspecies and interspecies nucleotide divergence), exists between species studied here. When our sequences were compared with the sequences of the conspecifics submitted from different geographic regions, nine cases of deep intraspecies nucleotide divergences were observed. In spite of this, NJ (Neighbour Joining) clustering analysis successfully discriminated all species. Observed cases of deep intraspecies nucleotide divergences certainly warrant further study.

Keywords

Western Ghats of India DNA barcoding Butterflies Nymphalidae Cytochrome c oxidase I 

Supplementary material

11033_2011_988_MOESM1_ESM.doc (322 kb)
Supplementary material 1 (DOC 373 kb)

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • S. S. Gaikwad
    • 1
  • H. V. Ghate
    • 1
  • S. S. Ghaskadbi
    • 2
  • M. S. Patole
    • 3
  • Y. S. Shouche
    • 3
  1. 1.Department of ZoologyModern CollegePuneIndia
  2. 2.Department of ZoologyUniversity of PunePuneIndia
  3. 3.National Center for Cell SciencesPuneIndia

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