DNA barcoding of nymphalid butterflies (Nymphalidae: Lepidoptera) from Western Ghats of India
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- Gaikwad, S.S., Ghate, H.V., Ghaskadbi, S.S. et al. Mol Biol Rep (2012) 39: 2375. doi:10.1007/s11033-011-0988-7
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We have checked the utility of DNA barcoding for species identification of nymphalid butterflies from Western Ghats of India by using 650 bp sequence of mitochondrial gene cytochrome c oxidase subunit I. Distinct DNA barcoding gap (i.e. difference between intraspecies and interspecies nucleotide divergence), exists between species studied here. When our sequences were compared with the sequences of the conspecifics submitted from different geographic regions, nine cases of deep intraspecies nucleotide divergences were observed. In spite of this, NJ (Neighbour Joining) clustering analysis successfully discriminated all species. Observed cases of deep intraspecies nucleotide divergences certainly warrant further study.