Molecular Biology Reports

, Volume 39, Issue 2, pp 817–824

MicroRNA regulated defense responses in Triticum aestivum L. during Puccinia graminis f.sp. tritici infection

  • Om Prakash Gupta
  • Vipin Permar
  • Vikas Koundal
  • Uday Dhari Singh
  • Shelly Praveen
Article

DOI: 10.1007/s11033-011-0803-5

Cite this article as:
Gupta, O.P., Permar, V., Koundal, V. et al. Mol Biol Rep (2012) 39: 817. doi:10.1007/s11033-011-0803-5

Abstract

Plants have evolved diverse mechanism to recognize pathogen attack and triggers defense responses. These defense responses alter host cellular function regulated by endogenous, small, non-coding miRNAs. To understand the mechanism of miRNAs regulated cellular functions during stem rust infection in wheat, we investigated eight different miRNAs viz. miR159, miR164, miR167, miR171, miR444, miR408, miR1129 and miR1138, involved in three different independent cellular defense response to infection. The investigation reveals that at the initiation of disease, accumulation of miRNAs might be playing a key role in hypersensitive response (HR) from host, which diminishes at the maturation stage. This suggests a possible host-fungal synergistic relation leading to susceptibility. Differential expression of these miRNAs in presence and absence of R gene provides a probable explanation of miRNA regulated R gene mediated independent pathways.

Keywords

miRNADefense responseSignalingHypersensitive response (HR)R gene

Supplementary material

11033_2011_803_MOESM1_ESM.doc (32 kb)
Supplementary data: List of miRNA and their primer sequence (online supplementary data) (DOC 32 kb)

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Om Prakash Gupta
    • 1
  • Vipin Permar
    • 2
  • Vikas Koundal
    • 2
  • Uday Dhari Singh
    • 2
  • Shelly Praveen
    • 2
  1. 1.Division of BiochemistryIndian Agricultural Research InstituteNew DelhiIndia
  2. 2.Division of Plant Pathology, Advanced Centre for Plant Virology Indian Agricultural Research InstituteNew DelhiIndia