Molecular Biology Reports

, Volume 39, Issue 1, pp 33–39

Shine-Dalgarno sequence of bacteriophage T4: GAGG prevails in early genes


DOI: 10.1007/s11033-011-0707-4

Cite this article as:
Malys, N. Mol Biol Rep (2012) 39: 33. doi:10.1007/s11033-011-0707-4


Translation initiation is governed by a limited number of mRNA sequence motifs within the translation initiation region (TIR). In bacteria and bacteriophages, one of the most important determinants is a Shine-Dalgarno (SD) sequence that base pairs with the anti-SD sequence GAUCACCUCCUUA localized in the 3′ end of 16S rRNA. This work assesses a diversity of TIR features in phage T4, focusing on the SD sequence, its spacing to the start codon and relationship to gene expression and essentiality patterns. Analysis shows that GAGG is predominant of all core SD motifs in T4 and its related phages, particularly in early genes. Possible implication of the RegB activity is discussed.


Translation initiationShine-Dalgarno sequencePhage T4T4-related phagesEarly genesRegB

Supplementary material

11033_2011_707_MOESM1_ESM.doc (32 kb)
Supplementary material 1 (DOC 32 kb)
11033_2011_707_MOESM2_ESM.ppt (413 kb)
Supplementary material 2 (PPT 413 kb)

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.Faculty of Life Sciences, MCISB, MIBUniversity of ManchesterManchesterUK