Molecular Biology Reports

, Volume 38, Issue 5, pp 3075–3082

Establishment and identification of the human multi-drug-resistant cholangiocarcinoma cell line QBC939/ADM

  • Zhi-Hua Liu
  • Yan-Ping He
  • Yukun Zhou
  • Peng Zhang
  • Huanlong Qin
Article

DOI: 10.1007/s11033-010-9975-7

Cite this article as:
Liu, ZH., He, YP., Zhou, Y. et al. Mol Biol Rep (2011) 38: 3075. doi:10.1007/s11033-010-9975-7
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Abstract

In this study, we aim to establish the human multi-drug-resistant cholangiocarcinoma cell line QBC939/ADM which can be grow and passaged steadily in 1 μg/ml concentration of adriamycin in appropriate medium. The human multi-drug-resistant cholangiocarcinoma cell line QBC939/ADM was established using the method of exposure to medium with adriamycin alternated between high and low concentration with gradually increasing concentration. Furthermore, QBC939 and QBC939/ADM were both treated with adriamycin, mitomycin and vindesine, and then detected by MTT assay, respectively. Growth cycle and intra-cellular concentrations of ADM within cells of each group were determined by flow cytometry. Expression levels of P-glycoprotein were detected by Western bolt and real-time PCR. Results showed that, compared with QBC939, the inhibitive rates of adriamycin, mitomycin and vindesine to QBC939/ADM were lower. Content of ADM in the QBC939/ADM was lower. Western bolt and real time PCR showed that P-glycoprotein in the QBC939/ADM group was over expressed. Therefore, QBC939/ADM was establish and identified as the multi-drug-resistant cell line, which can grow and be passaged steadily in 1 μg/ml concentration adriamycin in appropriate medium. And the multi-drug-resistant character of QBC939/ADM was indicated to be related to the over expression of P-glycoprotein induced by chemotherapeutic drugs.

Keywords

CholangiocarcinomaMultidrug-resistantP-glycoprotein

Supplementary material

11033_2010_9975_MOESM1_ESM.tif (3 mb)
Fig. S1Changes of cell shape for QBC939/ADM. a QBC939/ADM cells were transformed into multinucleated giant cells and shown obvious changes of various cell shapes, such as fusiform, polygon, triangle, oval, and irregular forms. Karyons of QBC939/ADM were also changed into large, oval, irregular and double karyotypes. b QBC939 cells, which have the ability of steady growth in 0.5 μg/ml concentration of ADM, showed similar shape with QBC939/ADM. c QBC939 cells, which have the ability of steady growth in 0.25 μg/ml concentration of ADM, showed similar shape with primary QBC939 cells. d Primary QBC939 cells (TIFF 3111 kb)

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Zhi-Hua Liu
    • 1
  • Yan-Ping He
    • 2
  • Yukun Zhou
    • 1
  • Peng Zhang
    • 1
  • Huanlong Qin
    • 1
  1. 1.Department of SurgeryShanghai Jiao Tong University Affiliated Sixth People’s HospitalShanghaiChina
  2. 2.Department of SurgeryShanxi Medical UniversityTaiyuanChina