Molecular Biology Reports

, Volume 38, Issue 8, pp 5125–5132

CTLA-4 exon 1 +49 polymorphism alone and in a haplotype with −318 promoter polymorphism may confer susceptibility to chronic HBV infection in Chinese Han patients

Authors

  • Shaoqiong Duan
    • Department of Infectious Diseases, First Affiliated Hospital, School of MedicineXi’an Jiaotong University
  • Guoyu Zhang
    • Department of Infectious Diseases, First Affiliated Hospital, School of MedicineXi’an Jiaotong University
  • Qunying Han
    • Department of Infectious Diseases, First Affiliated Hospital, School of MedicineXi’an Jiaotong University
  • Zhu Li
    • Department of Infectious Diseases, First Affiliated Hospital, School of MedicineXi’an Jiaotong University
    • Department of Infectious Diseases, First Affiliated Hospital, School of MedicineXi’an Jiaotong University
  • Jinghong Chen
    • Key Laboratory of Environment and Genes related to Diseases, Ministry of Education, Institute of Endemic Diseases, School of MedicineXi’an Jiaotong University
  • Yi Lv
    • Department of Hepatobiliary Surgery, First Affiliated Hospital, School of MedicineXi’an Jiaotong University
  • Na Li
    • Department of Infectious Diseases, First Affiliated Hospital, School of MedicineXi’an Jiaotong University
  • Yawen Wang
    • Department of Infectious Diseases, First Affiliated Hospital, School of MedicineXi’an Jiaotong University
  • Man Li
    • Department of Infectious Diseases, First Affiliated Hospital, School of MedicineXi’an Jiaotong University
  • Sai Lou
    • Department of Infectious Diseases, First Affiliated Hospital, School of MedicineXi’an Jiaotong University
  • Mingbo Yang
    • Department of Infectious Diseases, First Affiliated Hospital, School of MedicineXi’an Jiaotong University
  • Qianqian Zhu
    • Department of Infectious Diseases, First Affiliated Hospital, School of MedicineXi’an Jiaotong University
  • Fanfan Xing
    • Department of Infectious Diseases, First Affiliated Hospital, School of MedicineXi’an Jiaotong University
Article

DOI: 10.1007/s11033-010-0660-7

Cite this article as:
Duan, S., Zhang, G., Han, Q. et al. Mol Biol Rep (2011) 38: 5125. doi:10.1007/s11033-010-0660-7

Abstract

Cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) plays a pivotal role in regulating T cell activation, which is believably critical for the outcome of hepatitis B virus (HBV) infection. The expression and function of CTLA-4 may be affected by gene polymorphisms. This study investigated the influence of CTLA-4 polymorphisms on disease susceptibility in Chinese Han patients with chronic HBV infection. CTLA-4 +49A/G and −318C/T polymorphisms were evaluated by DNA amplification with polymerase chain reaction followed by the restriction fragment length polymorphism analysis. The patients with chronic HBV infection had higher frequencies of genotype AA and allele A of CTLA-4 +49A/G polymorphism. The haplotype +49A−318C was significantly over-represented (P < 0.001) and haplotype +49G−318C under-represented (P = 0.006) in the patients. The +49GG genotype was more frequent (P = 0.009) and +49A allele was less frequent in patients with lower ALT levels (P = 0.012) in HBeAg positive chronic hepatitis B. It is indicated that CTLA-4 +49A/G polymorphism alone and in a haplotype with −318C allele may confer susceptibility to chronic HBV infection in Chinese Han patients.

Keywords

Chronic HBV infectionCTLA-4Genetic polymorphismAssociation

Supplementary material

11033_2010_660_MOESM1_ESM.doc (35 kb)
Supplementary material 1 (DOC 35 kb)

Copyright information

© Springer Science+Business Media B.V. 2010