Article

Molecular Biology Reports

, Volume 38, Issue 7, pp 4257-4264

First online:

Analysis of variable sites between two complete South China tiger (Panthera tigris amoyensis) mitochondrial genomes

  • Wenping ZhangAffiliated withThe Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding
  • , Bisong YueAffiliated withThe Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, Sichuan University Email author 
  • , Xiaofang WangAffiliated withThe Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, Sichuan University
  • , Xiuyue ZhangAffiliated withThe Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, Sichuan University
  • , Zhong XieAffiliated withChinese Association of Zoological Gardens
  • , Nonglin LiuAffiliated withChinese Association of Zoological Gardens
  • , Wenyuan FuAffiliated withFujian Meihuashan Institute of South China Tiger Breeding
  • , Yaohua YuanAffiliated withShanghai Zoo
  • , Daqing ChenAffiliated withSuzhou Zoo
    • , Danghua FuAffiliated withNanchang Zoo
    • , Bo ZhaoAffiliated withChengdu Zoo
    • , Yuzhong YinAffiliated withChongqing Zoo
    • , Xiahui YanAffiliated withChangsha Zoo
    • , Xinjing WangAffiliated withGuangzhou Zoo
    • , Rongying ZhangAffiliated withShenzhen Wild Zoo
    • , Jie LiuAffiliated withGuiyang Wild Zoo
    • , Maoping LiAffiliated withLuoyang Wangcheng Garden
    • , Yao TangAffiliated withFuzhou Zoo
    • , Rong HouAffiliated withThe Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding
    • , Zhihe ZhangAffiliated withThe Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding Email author 

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Abstract

In order to investigate the mitochondrial genome of Panthera tigris amoyensis, two South China tigers (P25 and P27) were analyzed following 15 cymt-specific primer sets. The entire mtDNA sequence was found to be 16,957 bp and 17,001 bp long for P25 and P27 respectively, and this difference in length between P25 and P27 occurred in the number of tandem repeats in the RS-3 segment of the control region. The structural characteristics of complete P. t. amoyensis mitochondrial genomes were also highly similar to those of P. uncia. Additionally, the rate of point mutation was only 0.3% and a total of 59 variable sites between P25 and P27 were found. Out of the 59 variable sites, 6 were located in 6 different tRNA genes, 6 in the 2 rRNA genes, 7 in non-coding regions (one located between tRNA-Asn and tRNA-Tyr and six in the D-loop), and 40 in 10 protein-coding genes. COI held the largest amount of variable sites (9 sites) and Cytb contained the highest variable rate (0.7%) in the complete sequences. Moreover, out of the 40 variable sites located in 10 protein-coding genes, 12 sites were nonsynonymous.

Keywords

Complete mitochondrial genome Panthera tigris amoyensis Variable site