Molecular Biology Reports

, Volume 38, Issue 6, pp 3751–3756

Molecular cloning and expression analysis of a F-type lectin gene from Japanese sea perch (Lateolabrax japonicus)

  • Lihua Qiu
  • Liansheng Lin
  • Keng Yang
  • Hanhua Zhang
  • Jianzhu Li
  • Falin Zou
  • Shigui Jiang
Article

DOI: 10.1007/s11033-010-0490-7

Cite this article as:
Qiu, L., Lin, L., Yang, K. et al. Mol Biol Rep (2011) 38: 3751. doi:10.1007/s11033-010-0490-7

Abstract

The techniques of homology cloning and anchored PCR were used to clone the fucose-binding lectin (F-type lectin) gene from Japanese sea perch (Lateolabrax Japonicus). The full-length cDNA of sea perch F-lectin (JspFL) contained a 5′ untranslated region (UTR) of 39 bp, an ORF of 933 bp encoding a polypeptide of 310 amino acids with an estimated molecular mass of 10.82 kDa and a 3′ UTR of 332 bp. The searches for nucleotides and protein sequence similarities with BLAST analysis indicated that the deduced amino acid sequence of JspFL was homological to the Fucose-binding lectin in other fish species. In the JspFL deduced amino acid sequence, two tandem domains that exhibit the eel carbohydrate-recognition sequence motif were found. The temporal expressions of gene in the different tissues were measured by real-time PCR. And the mRNA expressions of the gene were constitutively expressed in tissues including spleen, head-kidney, liver, gill, and heart. The JspFL expression in spleen was different during the stimulated time point, 2 h later the expression level became up-regulated, and 6 h later the expression level became down-regulated. The result indicated that JspFL was constitutive and inducible expressed and could play a critical role in the host-pathogen interaction.

Keywords

F-type lectinCloningExpressionJapanese sea perch

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Lihua Qiu
    • 1
  • Liansheng Lin
    • 2
  • Keng Yang
    • 1
  • Hanhua Zhang
    • 1
  • Jianzhu Li
    • 1
  • Falin Zou
    • 1
  • Shigui Jiang
    • 1
  1. 1.Biotechnology and Aquiculture LaboratoryThe South China Sea Fisheries Research Institute, Chinese Academy of Fishery SciencesGuangzhouPeople’s Republic of China
  2. 2.Chinese Academy of Fishery SciencesBeijingPeople’s Republic of China