Article

Molecular Biology Reports

, Volume 38, Issue 4, pp 2485-2490

Differential effects of platelet rich plasma and washed platelets on the proliferation of mouse MSC cells

  • Jianmin DuanAffiliated withDepartment of Stomatology, Guangzhou General Hospital of Guangzhou Military Command
  • , Wei KuangAffiliated withDepartment of Stomatology, Guangzhou General Hospital of Guangzhou Military Command Email author 
  • , Jiali TanAffiliated withDepartment of Orthodontics, School of Stomatology, The Fourth Military Medical University
  • , Hongtao LiAffiliated withDepartment of Stomatology, Guangzhou General Hospital of Guangzhou Military Command
  • , Yi ZhangAffiliated withDepartment of Stomatology, Guangzhou General Hospital of Guangzhou Military Command
  • , Kikuchi HirotakaAffiliated withDivision of Endodontics, Department of Restorative and Biomaterials Sciences, Meikai University School of Dentistry
  • , Katayama TadashiAffiliated withDivision of Endodontics, Department of Restorative and Biomaterials Sciences, Meikai University School of Dentistry

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Abstract

Multipotent mesenchymal stem cell (MSC) therapies are being tested clinically for a variety of disorders. However, despite the remarkable clinical advancements in this field, most applications still use traditional culture media containing fetal bovine serum. Platelet-rich plasma (PRP) appears as a novel application for tissue engineering and its effect on bone healing is thought to be mainly dependent on the proliferation promoting function, with the molecular mechanisms largely unknown. In this study, mouse osteogenic progenitor mesenchymal stem cells (MSCs) were cultured in PRP or washed platelet (WPLT)-treated wells or in untreated wells, and analyzed on cycloxygenase 2 (COX2) expression (qRT-PCR), cell growth (MTT assay) and cell differentiation (alkaline phosphatase activity). The results showed that PRP and WPLT stimulated cell growth similarly in the first 6 days, together with the steady induction of COX2 and PGE2. 10 μmol/l celecoxib (an inhibitor of COX2) significantly inhibited the pro-proliferation effects. Interestingly, WPLT had stronger effects than PRP in proliferation at the later time points (6–9 days). ALP activity assay and collagen 1a expression revealed PRP had a mild but statistically significant pro-differentiation effect, while no obvious effects observed in WLPT group. In summary, PRP stimulates initial growth of MSCs in a COX2 partially dependent manner and the less obvious osteogenic differentiation promoting effects of WPLT strongly indicates WPLT rather than the PRP should be the optional choice for expanding MSCs in vitro for clinical use.

Keywords

Mesenchymal stem cells (MSCs) Platelet-rich plasma (PRP) Washed platelet (WPLT) Osteogenic differentiation