Molecular Biology Reports

, Volume 38, Issue 3, pp 1457–1462

Golgi phosphoprotein 2 (GOLPH2/GP73/GOLM1) interacts with secretory clusterin

Article

DOI: 10.1007/s11033-010-0251-7

Cite this article as:
Zhou, Y., Li, L., Hu, L. et al. Mol Biol Rep (2011) 38: 1457. doi:10.1007/s11033-010-0251-7

Abstract

Golgi phosphoprotein 2 (GOLPH2/GP73/GOLM1), a type-II Golgi transmembrane protein of unknown function, is up-regulated in many cancers. Its Golgi luminal domain is potentially the major functional domain. The goal of this study is to identify the proteins interacting with GOLPH2. Using secretory GOLPH2 (sGOLPH2, amino acid residues 55–401) as bait, secretory clusterin (sCLU) was identified as one interacting candidate by yeast two-hybrid screening, and the coiled-coil domain of GOLPH2 was found to be sufficient for interaction with sCLU. The interaction between GOLPH2 and sCLU was confirmed intracellularly and extracellularly. The intracellular co-localization of GOLPH2 and sCLU in Golgi was also shown. These results can help in understanding the biological and pathological significance of GOLPH2.

Keywords

GOLPH2GP73ClusterinProtein interactionCoiled-coil domain

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.State Key Laboratory of Respiratory Diseases, Laboratory of Viral ImmunologyGuangzhou Institute of Biomedicine and Health (GIBH), Chinese Academy of SciencesGuangzhouPeople’s Republic of China
  2. 2.University of Science and Technology of ChinaHefeiPeople’s Republic of China
  3. 3.Guangzhou Institute of Biomedicine and HealthGuangzhou Science ParkPeople’s Republic of China