Molecular Biology Reports

, Volume 38, Issue 2, pp 1425–1431

The porcine TBC1D1 gene: mapping, SNP identification, and association study with meat, carcass and production traits in Italian heavy pigs

Authors

    • DIPROVAL, Sezione di Allevamenti Zootecnici, Faculty of AgricultureUniversity of Bologna
  • Michela Colombo
    • DIPROVAL, Sezione di Allevamenti Zootecnici, Faculty of AgricultureUniversity of Bologna
  • Lucia Tognazzi
    • DIPROVAL, Sezione di Allevamenti Zootecnici, Faculty of AgricultureUniversity of Bologna
  • Emilio Scotti
    • DIPROVAL, Sezione di Allevamenti Zootecnici, Faculty of AgricultureUniversity of Bologna
  • Luca Buttazzoni
    • Associazione Nazionale Allevatori Suini (ANAS)
  • Stefania Dall’Olio
    • DIPROVAL, Sezione di Allevamenti Zootecnici, Faculty of AgricultureUniversity of Bologna
  • Roberta Davoli
    • DIPROVAL, Sezione di Allevamenti Zootecnici, Faculty of AgricultureUniversity of Bologna
  • Vincenzo Russo
    • DIPROVAL, Sezione di Allevamenti Zootecnici, Faculty of AgricultureUniversity of Bologna
Article

DOI: 10.1007/s11033-010-0247-3

Cite this article as:
Fontanesi, L., Colombo, M., Tognazzi, L. et al. Mol Biol Rep (2011) 38: 1425. doi:10.1007/s11033-010-0247-3

Abstract

TBC1D1 [TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1] is a Rab-GTPase-activating related protein implicated in regulating the trafficking of glucose transporter 4 (GLUT4 or SLC2A4) storage vesicles to the cell surface in response to insulin and AMPK-activating stimuli in skeletal muscle. Mutations in the human and mouse TBC1D1 genes confer risk of obesity or leanness. We identified five single nucleotide polymorphisms (SNPs) in the porcine TBC1D1 gene. One of them (FN677935:g.219G>A) was genotyped either by high resolution melting and PCR-RFLP analyses to study allele frequencies in a few pig breeds and evaluate association with meat production and carcass traits in five groups of sib-tested pigs of Italian Large White and Italian Duroc breeds. The g.219G>A SNP was associated (P < 0.05) with ham weight, back fat thickness and lean cuts content in Italian Large White and with visible intermuscular fat in Italian Duroc pigs.

Keywords

Association study Meat production Candidate gene Pig SNP TBC1D1

Copyright information

© Springer Science+Business Media B.V. 2010