Article

Molecular Biology Reports

, Volume 38, Issue 1, pp 379-385

First online:

Association of polymorphisms in the human IL-10 and IL-18 genes with rheumatoid arthritis

  • Binwu YingAffiliated withDepartment of Laboratory Medicine, West China Hospital, Sichuan University
  • , Yunying ShiAffiliated withDepartment of Nephrology, West China Hospital, Sichuan University
  • , Xiaofu PanAffiliated withDepartment of Laboratory Medicine, West China Hospital, Sichuan University
  • , Xingbo SongAffiliated withDepartment of Laboratory Medicine, West China Hospital, Sichuan University
  • , Zhunchun HuangAffiliated withDepartment of Laboratory Medicine, West China Hospital, Sichuan University
  • , Qian NiuAffiliated withDepartment of Laboratory Medicine, West China Hospital, Sichuan University
  • , Bei CaiAffiliated withDepartment of Laboratory Medicine, West China Hospital, Sichuan University
  • , Lanlan WangAffiliated withDepartment of Laboratory Medicine, West China Hospital, Sichuan University Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

The decrease of anti-inflammatory cytokine and increase of pro-inflammatory cytokine was observed in rheumatoid arthritis (RA). Interleukin-10 (IL-10), a potent anti-inflammatory cytokine, has been demonstrated to suppress joint swelling and deformation in RA animal model. Interleukin-18 (IL-18), a widely distributed pro-inflammatory cytokine, induces the production of IFN-γ, activate NK cells, and promote inflammation. Recent studies demonstrated that the serum IL-10 and IL-18 levels may be influenced by genetics and related to susceptibility to several autoimmune diseases. In the present study, using polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) and DNA sequencing techniques, we analyzed the genotype and allele distributions of two single nucleotide polymorphisms (SNP) loci in the promoter region of IL-10 and IL-18 genes (IL-10-592 A/C and IL-18-607 A/C loci, respectively). Our results indicated that IL-10-592 allelic and genotypic frequencies were significantly different between the RA patients and normal subjects (P < 0.05). In addition, significant differences of IL-10-592 allelic and genotypic frequencies were also detected between the patients with or without anti-cyclic citrullinated peptide antibody (anti-CCP) (P < 0.05). In contrast, allelic and genotypic frequencies of IL-18-607 did not show significant difference between RA patients and normal subjects (P > 0.05) or between anti-CCP-positive and anti-CCP-negative RA patients (P > 0.05). Furthermore, ELISA detection of IL-10 and IL-18 serum levels revealed that the genotype of IL-10-592 was associated with IL-10 serum level (P < 0.05), but the genotype and allele frequency of IL-18-607 was not associated with IL-18 serum level (P > 0.05). Taken together, our findings provide new insight for the polymorphism of IL-10 gene in the pathogenesis of RA.

Keywords

IL-10 IL-18 Single nucleotide polymorphisms Rheumatoid arthritis