Molecular Biology Reports

, Volume 38, Issue 1, pp 115–121

A genetic investigation of Korean mummies from the Joseon Dynasty

Authors

  • Na Young Kim
    • Department of Forensic Medicine and Brain Korea 21 Project for Medical ScienceYonsei University College of Medicine
  • Hwan Young Lee
    • Department of Forensic Medicine and Brain Korea 21 Project for Medical ScienceYonsei University College of Medicine
    • Human Identification Research CenterYonsei University
  • Myung Jin Park
    • Department of Forensic Medicine and Brain Korea 21 Project for Medical ScienceYonsei University College of Medicine
  • Woo Ick Yang
    • Department of Forensic Medicine and Brain Korea 21 Project for Medical ScienceYonsei University College of Medicine
    • Department of Forensic Medicine and Brain Korea 21 Project for Medical ScienceYonsei University College of Medicine
    • Human Identification Research CenterYonsei University
Article

DOI: 10.1007/s11033-010-0084-4

Cite this article as:
Kim, N.Y., Lee, H.Y., Park, M.J. et al. Mol Biol Rep (2011) 38: 115. doi:10.1007/s11033-010-0084-4

Abstract

Two Korean mummies (Danwoong-mirra and Yoon-mirra) found in medieval tombs in the central region of the Korean peninsula were genetically investigated by analysis of mitochondrial DNA (mtDNA), Y-chromosomal short tandem repeat (Y-STR) and the ABO gene. Danwoong-mirra is a male child mummy and Yoon-mirra is a pregnant female mummy, dating back about 550 and 450 years, respectively. DNA was extracted from soft tissues or bones. mtDNA, Y-STR and the ABO gene were amplified using a small size amplicon strategy and were analyzed according to the criteria of ancient DNA analysis to ensure that authentic DNA typing results were obtained from these ancient samples. Analysis of mtDNA hypervariable region sequence and coding region single nucleotide polymorphism (SNP) information revealed that Danwoong-mirra and Yoon-mirra belong to the East Asian mtDNA haplogroups D4 and M7c, respectively. The Y-STRs were analyzed in the male child mummy (Danwoong-mirra) using the AmpFlSTR® YfilerTM PCR Amplification Kit and an in-house Y-miniplex plus system, and could be characterized in 4 loci with small amplicon size. The analysis of ABO gene SNPs using multiplex single base extension methods revealed that the ABO blood types of Danwoong-mirra and Yoon-mirra are AO01 and AB, respectively. The small size amplicon strategy and the authentication process in the present study will be effectively applicable to future genetic analyses of various forensic and ancient samples.

Keywords

Korean mummyAncient DNAAuthenticityMitochondrial DNAY-chromosomal short tandem repeatABO blood type

Supplementary material

11033_2010_84_MOESM1_ESM.pdf (43 kb)
Supplementary material 1 (PDF 42 kb)
11033_2010_84_MOESM2_ESM.pdf (43 kb)
Supplementary material 2 (PDF 43 kb)

Copyright information

© Springer Science+Business Media B.V. 2010