Molecular Biology Reports

, Volume 37, Issue 7, pp 3541–3545

Assessment of microsatellite instability in head and neck cancer using consensus markers

  • Zubeyde Yalniz
  • Semra Demokan
  • Yusufhan Suoglu
  • Murat Ulusan
  • Nejat Dalay
Article

DOI: 10.1007/s11033-010-0001-x

Cite this article as:
Yalniz, Z., Demokan, S., Suoglu, Y. et al. Mol Biol Rep (2010) 37: 3541. doi:10.1007/s11033-010-0001-x

Abstract

Head and neck cancer is the sixth most common cancer in the world and one of the most lethal cancers. Microsatellite instability is an important characteristic of tumor cells and is observed both in presence and absence of mismatch repair gene mutations. The importance of microsatellite instability in head and neck cancer is not well established due to the lack of a consensus panel and selection of different markers, criteria and methodological variances. The main objective of this study was to investigate the performance of a consensus panel of microsatellite repeats by automated fragment analysis. Matched tumor and normal tissue samples from 99 patients were analyzed using five mononucleotide markers. Following PCR the amplified fragments were analyzed by capillary electrophoresis on an ABI 310 genetic analyzer. Microsatellite instability was observed in 26 patients. In 17 patients instability was detected at multiple loci. NR21 and BAT25 were the most frequently altered targets. These two mononucleotide markers could detect all samples displaying high-instability. In this study we describe a standardized fluorescent multiplex PCR combined with computerized analysis, which allows rapid and accurate analysis of a high number of samples and obviates the need to compare tumors with matching normal tissue.

Keywords

Microsatellite instability Head and neck cancer Mononucleotide markers 

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Zubeyde Yalniz
    • 1
  • Semra Demokan
    • 1
  • Yusufhan Suoglu
    • 2
  • Murat Ulusan
    • 2
  • Nejat Dalay
    • 1
  1. 1.Department of Basic OncologyI.U. Oncology InstituteCapa, IstanbulTurkey
  2. 2.Department of Otolaryngology, Istanbul Faculty of MedicineIstanbul UniversityIstanbulTurkey

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