Molecular Biology Reports

, Volume 37, Issue 7, pp 3413–3420

Genetic diversity of Iranian Aegilops tauschii Coss. using microsatellite molecular markers and morphological traits

  • Zahra Tahernezhad
  • Mohammad Javad Zamani
  • Mahmood Solouki
  • Mehdi Zahravi
  • Abbas Ali Imamjomeh
  • Mohammad Jafaraghaei
  • Mohammad Reza Bihamta
Article

DOI: 10.1007/s11033-009-9931-6

Cite this article as:
Tahernezhad, Z., Zamani, M.J., Solouki, M. et al. Mol Biol Rep (2010) 37: 3413. doi:10.1007/s11033-009-9931-6

Abstract

Aegilops tauschii Coss. is a diploid (2n = 2x = 14,DD) goat grass species which has contributed the D genome in common wheat. Genetic variations in 28 accessions of Aegilops tauschii belonged to different provinces of Iran, were evaluated using 16 morphological traits and 19 SSR markers. In number of spikelet per spike and plant height, there was a high variation in ssp. tauschii and ssp. strangulata respectively and for days to mature a low variation in both subspecies was found. Discriminant function analysis showed that 67.9% of original grouped cases correctly classified. Factor analysis indicated that three factor explain 66.49% of total variation. The three clusters revealed by the cluster analysis were not consistent with their geographical distributions. We determined 208 alleles using 19 microsatellites. Average of alleles for every locus was 10.94. The total average of PIC was 0.267. 2261 bands produced for total of genotypes and Chinese Spring had the highest bands (95 alleles). The range of similarity coefficients was between 0.23 and 0.73. Genotypes were clustered using UPGMA method. The accessions did not match according to morphological cluster and geographical regions. 51.2% of total variations were related to 9 principle components.

Keywords

Aegilops tauschiiGenetic diversityMorphological traitMicrosatellite

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Zahra Tahernezhad
    • 1
  • Mohammad Javad Zamani
    • 2
  • Mahmood Solouki
    • 3
  • Mehdi Zahravi
    • 4
  • Abbas Ali Imamjomeh
    • 3
  • Mohammad Jafaraghaei
    • 4
  • Mohammad Reza Bihamta
    • 5
  1. 1.Molecular Marker LabSeed and Plant Certification and Registration InstituteKarajIran
  2. 2.Seed and Plant Improvement InstituteKarajIran
  3. 3.Department of Agronomy & Plant BreedingUniversity of ZabolZabolIran
  4. 4.National Plant Gene Bank of IranKarajIran
  5. 5.Department of Agronomy and Plant breeding, Agriculture CollegeTehran UniversityKarajIran