Molecular Biology Reports

, Volume 37, Issue 7, pp 3183–3192

Molecular interaction of α-synuclein with tubulin influences on the polymerization of microtubule in vitro and structure of microtubule in cells

  • R. M. Zhou
  • Y. X. Huang
  • X. L. Li
  • C. Chen
  • Q. Shi
  • G. R. Wang
  • C. Tian
  • Z. Y. Wang
  • Y. Y. Jing
  • C. Gao
  • X. P. Dong
Article

DOI: 10.1007/s11033-009-9899-2

Cite this article as:
Zhou, R.M., Huang, Y.X., Li, X.L. et al. Mol Biol Rep (2010) 37: 3183. doi:10.1007/s11033-009-9899-2

Abstract

Microtubule dynamics is essential for many vital cellular processes such as in intracellular transport, metabolism, and cell division. Evidences demonstrate that α-synuclein may associate with microtubular cytoskeleton and its major component, tubulin. In the present study, the molecular interaction between α-synuclein and tubulin was confirmed by GST pull-down assay and co-immunoprecipitation. The interacting regions within α-synuclein with tubulin were mapped at the residues 60–100 of α-synuclein that is critical for the binding activity with tubulin. Microtubule assembly assays and sedimentation tests demonstrated that α-synuclein influenced the polymerization of tubulin in vitro, revealing an interacting region-dependent feature. Confocal microscopy detected that exposures of α-synuclein proteins inhibited microtubule formation in the cultured cells, with a length-dependent phenomenon. Our data highlight a potential role of α-synuclein in regulating the microtubule dynamics in neurons. The association of α-synuclein with tubulin may further provide insight into the biological and pathophysiological function of synuclein.

Keywords

α-SynucleinTubulinMolecular interactionMicrotubule dynamicsSynucleinopathies

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • R. M. Zhou
    • 1
  • Y. X. Huang
    • 1
  • X. L. Li
    • 1
  • C. Chen
    • 1
  • Q. Shi
    • 1
  • G. R. Wang
    • 1
  • C. Tian
    • 1
  • Z. Y. Wang
    • 1
  • Y. Y. Jing
    • 1
  • C. Gao
    • 1
  • X. P. Dong
    • 1
  1. 1.State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and PreventionBeijingPeople’s Republic of China