Molecular Biology Reports

, Volume 37, Issue 3, pp 1627–1632

Curcumin decreases the expression of Pokemon by suppressing the binding activity of the Sp1 protein in human lung cancer cells

  • Jiajun Cui
  • Xianfeng Meng
  • Xudong Gao
  • Guangxuan Tan
Article

DOI: 10.1007/s11033-009-9575-6

Cite this article as:
Cui, J., Meng, X., Gao, X. et al. Mol Biol Rep (2010) 37: 1627. doi:10.1007/s11033-009-9575-6

Abstract

Pokemon, which stands for POK erythroid myeloid ontogenic factor, can regulate expression of many genes and plays an important role in tumorigenesis. Curcumin, a natural and non-toxic yellow compound, has capacity for antioxidant, free radical scavenger, anti-inflammatory properties. Recent studies shows it is a potential inhibitor of cell proliferation in a variety of tumour cells. To investigate whether curcumin can regulate the expression of Pokemon, a series of experiments were carried out. Transient transfection experiments demonstrated that curcumin could decrease the activity of the Pokemon promoter. Western blot analysis suggested that curcumin could significantly decrease the expression of the Pokemon. Overexpression of Sp1 could enhance the activity of the Pokemon promoter, whereas knockdown of Sp1 could decrease its activity. More important, we also found that curcumin could decrease the expression of the Pokemon by suppressing the stimulation of the Sp1 protein. Therefore, curcumin is a potential reagent for tumour therapy which may target Pokemon.

Keywords

PokemonCurcuminPromoterSp1Expression

Abbreviations

Sp1

Specificity protein 1

ChIP

Chromatin immunoprecipitation assay

GAPDH

Glyceraldehydes-3-phsphate dehydrogenase

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Jiajun Cui
    • 1
    • 3
  • Xianfeng Meng
    • 2
  • Xudong Gao
    • 4
  • Guangxuan Tan
    • 1
  1. 1.Zhoukou Normal UniversityZhoukouPeople’s Republic of China
  2. 2.Tanshan Medical UniversityBeijingPeople’s Republic of China
  3. 3.Beijing Institute of BiotechnologyBeijingPeople’s Republic of China
  4. 4.307 Hospital of PLABeijingPeople’s Republic of China