Molecular Biology Reports

, Volume 36, Issue 8, pp 2249–2258

PVAS3, a class-II ubiquitous asparagine synthetase from the common bean (Phaseolus vulgaris)

  • Esmeralda Parra-Peralbo
  • Manuel Pineda
  • Miguel Aguilar
Article

DOI: 10.1007/s11033-008-9441-y

Cite this article as:
Parra-Peralbo, E., Pineda, M. & Aguilar, M. Mol Biol Rep (2009) 36: 2249. doi:10.1007/s11033-008-9441-y

Abstract

A gene encoding a putative asparagine synthetase (AS; EC 6.3.5.4) has been isolated from common bean (Phaseolus vulgaris). A 2.4 kb cDNA clone of this gene (PVAS3) encodes a protein of 570 amino acids with a predicted molecular mass of 64,678 Da, an isoelectric point of 6.45, and a net charge of −5.9 at pH 7.0. The PVAS3 protein sequence conserves all the amino acid residues that are essential for glutamine-dependent AS, and PVAS3 complemented an E. coli asparagine auxotroph, that demonstrates that it encodes a glutamine-dependent AS. PVAS3 displayed significant similarity to other AS. It showed the highest similarity to soybean SAS3 (92.9% identity), rice AS (73.7% identity), Arabidopsis ASN2 (73.2%) and sunflower HAS2 (72.9%). A phylogenetic analysis revealed that PVAS3 belongs to class-II asparagine synthetases. Expression analysis by real-time RT-PCR revealed that PVAS3 is expressed ubiquitously and is not repressed by light.

Keywords

Asparagine synthetase Legume Phaseolus vulgaris 

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Esmeralda Parra-Peralbo
    • 1
  • Manuel Pineda
    • 1
  • Miguel Aguilar
    • 1
  1. 1.Departamento de Botánica, Ecología y Fisiología Vegetal, Instituto Andaluz de BiotecnologíaUniversidad de CórdobaCórdobaSpain