Molecular Biology Reports

, 36:1637

A cyclophilin A inducible expressed in gonad of zhikong scallop Chlamys farreri

  • Xiaoyan Song
  • Lingling Wang
  • Linsheng Song
  • Jianmin Zhao
  • Huan Zhang
  • Peilin Zheng
  • Limei Qiu
  • Xiaolin Liu
  • Longtao Wu
Article

DOI: 10.1007/s11033-008-9363-8

Cite this article as:
Song, X., Wang, L., Song, L. et al. Mol Biol Rep (2009) 36: 1637. doi:10.1007/s11033-008-9363-8

Abstract

Cyclophilin A (CypA), a receptor for the immunosuppressive agent cyclosporin A (CsA), is a cis–trans peptidyl-prolyl isomerase (PPIase) which accelerates the cistrans isomerization of prolyl-peptide bonds, interacts with a variety of proteins and therefore regulates their activities. One CypA (designated CfCypA) cDNA was cloned from Chlamys farreri by expressed sequence tag (EST) and rapid amplification of cDNA ends (RACE) techniques. The full-length cDNA of CfCypA consisted of 1,248 nucleotides with a canonical polyadenylation signal sequence AATAAA, a poly (A) tail, and an open reading frame (ORF) of 495 nucleotides encoding a polypeptide of 164 amino acids. The deduced amino acid sequence shared high similarity with CypA from the other species, indicating that CfCypA should be a new member of the CypA family. Quantitative real-time (RT) PCR was employed to assess the mRNA expression of CfCypA in various tissues and its temporal expression in haemocytes and gonad of scallops challenged with Vibrio anguillarum. The mRNA transcripts of CfCypA could be detected in all the examined tissues with highest expression level in gonad. After bacterial challenge, the expression level of CfCypA was almost unchanged in haemocytes, but up-regulated in gonad and increased to the peak (22.59-fold; < 0.05) at 4 h post-injection, and then dropped to the original level at 8 h post-injection. These results indicated that CfCypA was constitutive expressed in haemocytes, but could be induced in gonad, and perhaps played a critical role in response to the bacterial challenge in gonad.

Keywords

Scallop Chlamys farreriCyclophilin AmRNA expressionQuantitative real-time PCRBacteria challenge

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Xiaoyan Song
    • 1
    • 2
  • Lingling Wang
    • 2
  • Linsheng Song
    • 2
  • Jianmin Zhao
    • 2
  • Huan Zhang
    • 2
  • Peilin Zheng
    • 2
  • Limei Qiu
    • 2
  • Xiaolin Liu
    • 1
  • Longtao Wu
    • 2
  1. 1.College of Animal Science and TechnologyNorthwest A&F UniversityYanglingChina
  2. 2.Institute of OceanologyChinese Academy of SciencesQingdaoChina