Article

Molecular Biology Reports

, Volume 36, Issue 6, pp 1637-1645

A cyclophilin A inducible expressed in gonad of zhikong scallop Chlamys farreri

  • Xiaoyan SongAffiliated withCollege of Animal Science and Technology, Northwest A&F UniversityInstitute of Oceanology, Chinese Academy of Sciences
  • , Lingling WangAffiliated withInstitute of Oceanology, Chinese Academy of Sciences
  • , Linsheng SongAffiliated withInstitute of Oceanology, Chinese Academy of Sciences Email author 
  • , Jianmin ZhaoAffiliated withInstitute of Oceanology, Chinese Academy of Sciences
  • , Huan ZhangAffiliated withInstitute of Oceanology, Chinese Academy of Sciences
  • , Peilin ZhengAffiliated withInstitute of Oceanology, Chinese Academy of Sciences
  • , Limei QiuAffiliated withInstitute of Oceanology, Chinese Academy of Sciences
  • , Xiaolin LiuAffiliated withCollege of Animal Science and Technology, Northwest A&F University
  • , Longtao WuAffiliated withInstitute of Oceanology, Chinese Academy of Sciences

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Abstract

Cyclophilin A (CypA), a receptor for the immunosuppressive agent cyclosporin A (CsA), is a cis–trans peptidyl-prolyl isomerase (PPIase) which accelerates the cistrans isomerization of prolyl-peptide bonds, interacts with a variety of proteins and therefore regulates their activities. One CypA (designated CfCypA) cDNA was cloned from Chlamys farreri by expressed sequence tag (EST) and rapid amplification of cDNA ends (RACE) techniques. The full-length cDNA of CfCypA consisted of 1,248 nucleotides with a canonical polyadenylation signal sequence AATAAA, a poly (A) tail, and an open reading frame (ORF) of 495 nucleotides encoding a polypeptide of 164 amino acids. The deduced amino acid sequence shared high similarity with CypA from the other species, indicating that CfCypA should be a new member of the CypA family. Quantitative real-time (RT) PCR was employed to assess the mRNA expression of CfCypA in various tissues and its temporal expression in haemocytes and gonad of scallops challenged with Vibrio anguillarum. The mRNA transcripts of CfCypA could be detected in all the examined tissues with highest expression level in gonad. After bacterial challenge, the expression level of CfCypA was almost unchanged in haemocytes, but up-regulated in gonad and increased to the peak (22.59-fold; < 0.05) at 4 h post-injection, and then dropped to the original level at 8 h post-injection. These results indicated that CfCypA was constitutive expressed in haemocytes, but could be induced in gonad, and perhaps played a critical role in response to the bacterial challenge in gonad.

Keywords

Scallop Chlamys farreri Cyclophilin A mRNA expression Quantitative real-time PCR Bacteria challenge