Molecular Biology Reports

, Volume 36, Issue 2, pp 381–388

Inhibition on Hepatitis B virus in vitro of recombinant MAP30 from bitter melon

  • Jian Ming Fan
  • Qiao Zhang
  • Jun Xu
  • Sha Zhu
  • Tao Ke
  • De Fu Gao
  • Yu Bao Xu
Article

DOI: 10.1007/s11033-007-9191-2

Cite this article as:
Fan, J.M., Zhang, Q., Xu, J. et al. Mol Biol Rep (2009) 36: 381. doi:10.1007/s11033-007-9191-2

Abstract

The gene encoding MAP30 protein was cloned from bitter melon and recombinant MAP30 was expressed and purified. The human hepatoma G2.2.15 cells were exposed to different concentrations of MAP30. MTT assay was used to evaluate the cytotoxicity of the drugs and real-time PCR and Southern hybridization were applied to quantify extracellular HBV DNA and replicative intermediates intracellular and cccDNA in nucleus. HBsAg and HBeAg were assessed by enzyme-linked immunosorbent assay (ELISA). The results showed that exposure of HepG2.2.15 cells to MAP30 resulted in inhibition of HBV DNA replication and HBsAg secretion. After exposed to three different concentrations of MAP30 for 2, 4, 6, and 8 days respectively, the inhibition rates of extracellular HBV DNA, HBsAg, and HBeAg of each concentration decreased significantly (P < 0.05). After 9 days of treatment, the inhibition rates of extracellular HBV DNA of the different concentrations differed greatly (P < 0.001). The MAP30 could inhibit the production of HBV (P < 0.01) dose-dependently. The expression of HBsAg was significantly decreased by MAP30 dose-dependently (P < 0.001) and time-dependently (P < 0.001). Lower dose of MAP30 (8.0 μg/ml) could inhibit the expression of HBsAg and HBeAg.

Keywords

Bitter melonHBV DNAHepG2.2.15 cellsMAP30MTTReplicative intermediates

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Jian Ming Fan
    • 1
  • Qiao Zhang
    • 1
  • Jun Xu
    • 2
  • Sha Zhu
    • 3
  • Tao Ke
    • 4
  • De Fu Gao
    • 5
  • Yu Bao Xu
    • 1
  1. 1.The Laboratory of Toxicology, College of Public HealthZhengzhou UniversityZhengzhouChina
  2. 2.School of Life SciencesHenan Agricultural UniversityZhengzhouChina
  3. 3.Department of Microbiology and Immunology, College of Basic Medical SciencesZhengzhou UniversityZhengzhouChina
  4. 4.School of Life SciencesNanYang Normal UniversityNanYangChina
  5. 5.College of Basic Medical SciencesHenan Institute of Traditional Chinese MedicineZhengzhouChina