Molecular Biology Reports

, Volume 36, Issue 1, pp 207–214

Cloning and characterization of the 14-3-3 protein gene from the halotolerant alga Dunaliella salina

  • Tianyun Wang
  • Lexun Xue
  • Xiang Ji
  • Jie Li
  • Yafeng Wang
  • Yingcai Feng
Article

DOI: 10.1007/s11033-007-9168-1

Cite this article as:
Wang, T., Xue, L., Ji, X. et al. Mol Biol Rep (2009) 36: 207. doi:10.1007/s11033-007-9168-1

Abstract

Previous studies have demonstrated that 14-3-3 proteins exist in all the eukaryotic organisms studied; however, studies on the 14-3-3 proteins have not been involved in the halotolerant, unicellular green alga Dunaliella salina so far. In the present study, a cDNA encoding 14-3-3 protein of D. salina was cloned and sequenced by PCR and rapid amplification of cDNA end (RACE) technique based on homologous sequences of the 14-3-3 proteins found in other organisms. The cloned cDNA of 1485 bp in length had a 29.2 kDa of molecular weight and contained a 774 bp of open reading frame encoding a polypeptide of 258 amino acids. Like the other 14-3-3 proteins, the deduced amino acid sequences of the D. salina 14-3-3 protein also contained two putative phosphorylation sites within the N-terminal region (positions 62 and 67). Furthermore, an EF hand motif characteristic for Ca2+-binding sites was located within the C-terminal part of this polypeptide (positions 208–219). Analysis of bioinformatics revealed that the 14-3-3 protein of D. salina shared homology with that of other organisms. Real-time quantitative PCR demonstrated that expression of the 14-3-3 protein gene is cell cycle-dependent.

Keywords

14-3-3 ProteinDunaliella salinaMolecular evolution

Abbreviations

ORF

Open reading frame

RACE

Rapid amplification of cDNA ends

RT-PCR

Reverse transcriptase-polymerase chain reaction

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Tianyun Wang
    • 1
    • 2
  • Lexun Xue
    • 1
  • Xiang Ji
    • 1
  • Jie Li
    • 1
  • Yafeng Wang
    • 1
  • Yingcai Feng
    • 1
  1. 1.Laboratory for Cell BiologyZhengzhou UniversityZhengzhouP.R. China
  2. 2.Department of Biochemistry and Molecular BiologyXinxiang Medical UniversityXinxiangP.R. China