Molecular Biology Reports

, Volume 35, Issue 4, pp 589–594

Overproduction of mouse estrogen receptor alpha-ligand binding domain decreases bacterial growth

Authors

  • Swati Ghosh
    • Biochemistry and Molecular Biology Laboratory, Center of Advanced Study in ZoologyBanaras Hindu University
    • Biochemistry and Molecular Biology Laboratory, Center of Advanced Study in ZoologyBanaras Hindu University
Article

DOI: 10.1007/s11033-007-9128-9

Cite this article as:
Ghosh, S. & Thakur, M.K. Mol Biol Rep (2008) 35: 589. doi:10.1007/s11033-007-9128-9

Abstract

Escherichia coli (E. coli) is the most widely used prokaryotic host system for the synthesis of recombinant proteins. The overproduction of recombinant proteins is sometimes lethal to the host cells. In the present study, we expressed the ligand binding domain (LBD) of mouse estrogen receptor alpha (mouse ERα) using an expression vector (pIVEX) in E. coli BL21(DE3) and examined the effect of production of this protein on bacterial growth. The expressed protein was immunologically detected as a 30 kD histidine-tagged protein in the soluble part of the bacterial lysate. The overproduction of mouse ERα-LBD, as reflected by total protein content and expression pattern, resulted in the decrease of bacterial growth.

Keywords

Estrogen receptor alphaLigand binding domainProteinOverproductionBacterial growthHost cell

Copyright information

© Springer Science+Business Media B.V. 2007