Molecular Biology Reports

, Volume 35, Issue 2, pp 179–187

A human homolog of mouse Lbh gene, hLBH, expresses in heart and activates SRE and AP-1 mediated MAPK signaling pathway

Authors

  • Jianping Ai
    • The Center For Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life SciencesHunan Normal University
  • Yuequn Wang
    • The Center For Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life SciencesHunan Normal University
  • Kunrong Tan
    • The Center For Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life SciencesHunan Normal University
  • Yun Deng
    • The Center For Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life SciencesHunan Normal University
  • Na Luo
    • The Center For Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life SciencesHunan Normal University
  • Wuzhou Yuan
    • The Center For Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life SciencesHunan Normal University
  • Zequn Wang
    • The Center For Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life SciencesHunan Normal University
  • Yongqing Li
    • The Center For Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life SciencesHunan Normal University
  • Ying Wang
    • The Center For Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life SciencesHunan Normal University
  • Xiaoyang Mo
    • The Center For Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life SciencesHunan Normal University
  • Chuanbing Zhu
    • The Center For Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life SciencesHunan Normal University
  • Zhaochu Yin
    • The Center For Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life SciencesHunan Normal University
    • The Center For Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life SciencesHunan Normal University
    • The Center For Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life SciencesHunan Normal University
Article

DOI: 10.1007/s11033-007-9068-4

Cite this article as:
Ai, J., Wang, Y., Tan, K. et al. Mol Biol Rep (2008) 35: 179. doi:10.1007/s11033-007-9068-4

Abstract

It has been reported that mouse Lbh (limb-bud and heart) can regulate cardiac gene expression by modulating the combinatorial activities of key cardiac transcription factors, as well as their individual functions in cardiogenesis. Here we report the cloning and characterization of the human homolog of mouse Lbh gene, hLBH, from a human embryonic heart cDNA library. The cDNA of hLBH is 2927 bp long, encoding a protein product of 105 amino acids. The protein is highly conserved in evolution across different species from zebra fish, to mouse, to human. Northern blot analysis indicates that a 2.9 kb transcript specific for hLBH is most abundantly expressed in both embryonic and adult heart tissue. In COS-7 cells, hLBH proteins are localized to both the nucleus and the cytoplasm. hLBH is a transcription activator when fused to Gal-4 DNA-binding domain. Deletion analysis indicates that both the N-terminal containing proline-dependent serine/threonine kinase group and the C-terminal containing ERK D-domain motif are required for transcriptional activation. Overexpression of hLBH in COS-7 cells activates the transcriptional activities of activator protein-1 (AP-1) and serum response element (SRE). These results suggest that hLBH proteins may act as a transcriptional activator in mitogen-activated protein kinase signaling pathway to mediate cellular functions.

Keywords

hLBHTranscription factorGene regulationSREAP-1

Abbreviations

DMEM

Dulbecco’s Modified Eagle Medium

DAPI

4′,6′-diamidino-2-phenylindole hydrochloride

MAPK

Mitogen-activated protein kinase

MAPKK, MKK or MEK

MAPK kinase

MAPKKK or MEKK

A MAPKK kinase or MEK kinase

SRE

Serum response element

AP-1

Activation protein 1

Copyright information

© Springer Science+Business Media, Inc. 2007