Molecular Biology Reports

, Volume 32, Issue 4, pp 247–255

Identification of a New Transcript Specifically Expressed in Mouse Spermatocytes: mmrp2

  • Shanye Gu
  • Jiarui Hu
  • Ping Song
  • Wuming Gong
  • Ming Guo
Article

DOI: 10.1007/s11033-005-3005-1

Cite this article as:
Gu, S., Hu, J., Song, P. et al. Mol Biol Rep (2005) 32: 247. doi:10.1007/s11033-005-3005-1

Abstract

In an effort to examine the molecular basis of gametogenesis, we screened Riken cDNA datebase and the clone 4930481F22 that is expressed preponderantly in mouse testis was identified. In the course of the research, a new isoform of 4930481F22 clone was found, isolated from mouse testis and sequenced. It only lacks the 7th exon of 4930481F22 transcript. The new isoform only has 837 bp and encodes a putative 28.4 kDa protein. We investigated the expression pattern at the mRNA level by RT-PCR and in situ hybridization in testis. The new isoform was only expressed in the gonad, where it began to be detected at day 8 after birth. In situ hybridization proved that the new isoform mostly expressed in spermatocytes. The structure of the predicted protein and the expression pattern of the mRNA suggest that the new isoform could have an important role in meiosis. We temporarily named it mmrp 2 (Mouse Meiosis Related Protein 2).

Keywords

gonad-specificin situ hybridizationRT-PCRsequence analysisspermatocyte

Copyright information

© Springer 2005

Authors and Affiliations

  • Shanye Gu
    • 1
  • Jiarui Hu
    • 2
  • Ping Song
    • 1
  • Wuming Gong
    • 1
  • Ming Guo
    • 2
  1. 1.Laboratory of Molecular Genetic and Developmental Biology, College of Life ScienceWuhan UniversityWuhanChina
  2. 2.Department of Gynecology and Obstetrics, Zhongnan HospitalWuhan UniversityWuhanChina