Molecular Breeding

, Volume 29, Issue 1, pp 43–52

Characterization of a cell wall invertase gene TaCwi-A1 on common wheat chromosome 2A and development of functional markers

  • Dongyun Ma
  • Jun Yan
  • Zhonghu He
  • Ling Wu
  • Xianchun Xia
Article

DOI: 10.1007/s11032-010-9524-z

Cite this article as:
Ma, D., Yan, J., He, Z. et al. Mol Breeding (2012) 29: 43. doi:10.1007/s11032-010-9524-z

Abstract

Cell wall invertase (CWI) is a critical enzyme for sink tissue development and carbon partition, and has a high association with kernel weight. Characterization of Cwi genes and development of functional markers are of importance for marker-assisted selection in wheat breeding. In the present study, the full-length genomic DNA sequence of a Cwi gene located on wheat chromosome 2A, designated TaCwi-A1, was characterized by in silico cloning and experimental validation. TaCwi-A1 comprises seven exons and six introns, with 3,676 bp in total, and an open reading frame (ORF) of 1,767 bp. A pair of complementary dominant markers, CWI21 and CWI22, was developed based on allelic variations at the TaCwi-A1 locus. A 404-bp PCR fragment was amplified by CWI21 in varieties with lower kernel weights, whereas a 402-bp fragment was generated by CWI22 in the varieties with higher kernel weights. The markers CWI21 and CWI22 were located on chromosome 2AL using a F2:3 population from a cross Doumai/Shi 4185, and a set of Chinese Spring nullisomic–tetrasomic lines. They were linked to the SSR locus Xbarc15-2AL with a genetic distance of 10.9 cM. QTL analysis indicated that TaCwi-A1 could explain 4.8% of phenotypic variance for kernel weight over 2 years. Two sets of Chinese landraces and two sets of commercial wheat varieties were used to validate the association of CWI21 and CWI22 with kernel weight. The results indicated that the functional markers CWI21 and CWI22 were closely related to kernel weight and could be used in wheat breeding for improving grain yield.

Keywords

Allelic variationCwi geneKernel weightQTLTriticum aestivum

Supplementary material

11032_2010_9524_MOESM1_ESM.docx (38 kb)
Supplementary material 1 (DOCX 38 kb)

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Dongyun Ma
    • 1
    • 2
  • Jun Yan
    • 3
  • Zhonghu He
    • 1
    • 4
  • Ling Wu
    • 1
    • 5
  • Xianchun Xia
    • 1
  1. 1.Institute of Crop Science, National Wheat Improvement Center/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS)BeijingChina
  2. 2.National Wheat Engineering Research CenterHenan Agricultural UniversityZhengzhouChina
  3. 3.Cotton Research Institute, Chinese Academy of Agricultural Sciences (CAAS)AnyangChina
  4. 4.CIMMYT China OfficeBeijingChina
  5. 5.Crop Research Institute, Sichuan Academy of Agricultural SciencesChengduChina