Article

Molecular Breeding

, Volume 28, Issue 1, pp 117-126

First online:

Molecular markers for tracking variation in lipoxygenase activity in wheat breeding

  • Hongwei GengAffiliated withCollege of Agronomy, Xingjiang Agricultural UniversityInstitute of Crop Science, National Wheat Improvement Center/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS)
  • , Yan ZhangAffiliated withInstitute of Crop Science, National Wheat Improvement Center/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS)
  • , Zhonghu HeAffiliated withInstitute of Crop Science, National Wheat Improvement Center/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS)International Maize and Wheat Improvement Center (CIMMYT) China Office Email author 
  • , Liping ZhangAffiliated withBeijing Engineering and Technique Research Center of Hybrid Wheat, Beijing Academy of Agricultural and Forestry Sciences
  • , Rudi AppelsAffiliated withCentre for Comparative Genomics, Murdoch University
  • , Yanying QuAffiliated withCollege of Agronomy, Xingjiang Agricultural University
  • , Xianchun XiaAffiliated withInstitute of Crop Science, National Wheat Improvement Center/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS) Email author 

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Abstract

Lipoxygenase (LOX) activity is an important factor determining the color of flour and end-use products of wheat. In the present study, quantitative trait loci (QTL) for LOX activity in common wheat were mapped using 71 doubled haploid (DH) lines derived from a Zhongyou 9507 × CA9632 cross, and SSR markers. Two QTL, QLpx.caas.1AL and QLpx.caas-4B, were identified on chromosomes 1AL and 4B, closely associated with LOX activity. The SSR loci Xwmc312 and Xgwm251 proved to be diagnostic and explained 13.4–25.2% of the phenotypic variance for the 1AL locus and 14.3–27.0% for the 4B locus across four environments. The SSR markers Xgwm251 and Xwmc312 were validated across 198 Chinese wheat cultivars and advanced lines and showed highly significant (P < 0.01) association with LOX activity. We further established a multiplexed PCR with SSR marker combination Xwmc312/Xgwm251 to test these wheat cultivars and advanced lines. The results suggested that the marker combination Xwmc312/Xgwm251 is efficient and reliable for evaluating LOX activity and can be used in marker-assisted selection (MAS) for targeting flour color attributes to noodle and other wheat-based products.

Keywords

Triticum aestivum L. Lipoxygenase activity Molecular marker QTL Multiplex PCR