Molecular Breeding

, Volume 26, Issue 2, pp 357–370

Recombination is suppressed in an alien introgression in peanut harboring Rma, a dominant root-knot nematode resistance gene

  • Ervin D. Nagy
  • Ye Chu
  • Yufang Guo
  • Sameer Khanal
  • Shunxue Tang
  • Yan Li
  • Weibo B. Dong
  • Patricia Timper
  • Christopher Taylor
  • Peggy Ozias-Akins
  • C. Corley Holbrook
  • Vadim Beilinson
  • Niels C. Nielsen
  • H. Thomas Stalker
  • Steven J. Knapp
Article

DOI: 10.1007/s11032-010-9430-4

Cite this article as:
Nagy, E.D., Chu, Y., Guo, Y. et al. Mol Breeding (2010) 26: 357. doi:10.1007/s11032-010-9430-4

Abstract

Rma, a dominant root-knot nematode resistance gene introduced into tetraploid peanut (Arachis hypogaea) from a synthetic allotetraploid donor (TxAG-6), has been widely deployed in modern cultivars. The genomic location and borders of the alien chromosome segment introgressed from TxAG-6 into NemaTAM (a BC7-derived introgression line) and other modern cultivars carrying Rma have not been genetically mapped, and resistance gene candidates (RGCs) have not been identified for Rma. Our study focused on densely populating the alien introgression with codominant DNA markers, identifying and mapping the borders of the alien introgression carried by NemaTAM, and identifying RGCs for Rma. Altogether, 2,847 simple sequence repeat (SSR) and 380 single strand conformational polymorphism (SSCP) markers were screened for linkage to Rma-247 of the SSCP markers targeted 202 nucleotide binding site (NBS) leucine-rich repeat (LRR) and other resistance (R) gene homologs (75 were identified by mining a peanut EST database). SSR, NBS-LRR, and Ser/Thr receptor-like protein loci within the alien introgression co-segregated with Rma in an F4 population (Gregory × Tifguard) and were tightly linked and spanned 3.4 cM in an F5 population (NemaTAM × GP-NC-WS-14). By comparative mapping in the A-genome progenitor of peanut (A. duranensis), Rma was discovered to have been introduced on an interstitial alien chromosome segment spanning one-third to one-half of chromosome 9A. Numerous codominant DNA markers were identified for finer mapping of Rma, shortening the alien introgression harboring Rma by marker-assisted selection, and introducing novel root-knot nematode R-genes into peanut by targeting syntenic segments on chromosomes 9A and 9B in wild diploid donors.

Keywords

Arachis Meloidogyne Marker-assisted selection Fabaceae Nucleotide binding site leucine-rich repeat Receptor-like kinase 

Supplementary material

11032_2010_9430_MOESM1_ESM.pdf (355 kb)
Supplementary material 1 (PDF 354 kb)
11032_2010_9430_MOESM2_ESM.pdf (1.1 mb)
Supplementary material 2 (PDF 1141 kb)
11032_2010_9430_MOESM3_ESM.xls (108 kb)
Supplementary material 3 (XLS 108 kb)
11032_2010_9430_MOESM4_ESM.pdf (20 kb)
Supplementary material 4 (PDF 19 kb)
11032_2010_9430_MOESM5_ESM.pdf (24 kb)
Supplementary material 5 (PDF 24 kb)
11032_2010_9430_MOESM6_ESM.xls (340 kb)
Supplementary material 6 (XLS 339 kb)

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Ervin D. Nagy
    • 1
  • Ye Chu
    • 2
  • Yufang Guo
    • 1
  • Sameer Khanal
    • 1
  • Shunxue Tang
    • 1
  • Yan Li
    • 1
  • Weibo B. Dong
    • 3
  • Patricia Timper
    • 4
  • Christopher Taylor
    • 1
  • Peggy Ozias-Akins
    • 2
  • C. Corley Holbrook
    • 4
  • Vadim Beilinson
    • 5
  • Niels C. Nielsen
    • 5
  • H. Thomas Stalker
    • 5
  • Steven J. Knapp
    • 1
  1. 1.Institute of Plant Breeding, Genetics, and GenomicsUniversity of GeorgiaAthensUSA
  2. 2.Department of HorticultureUniversity of GeorgiaTiftonUSA
  3. 3.Department of Plant PathologyUniversity of GeorgiaTiftonUSA
  4. 4.U.S. Department of AgricultureAgricultural Research ServiceTiftonUSA
  5. 5.Department of Crop ScienceNorth Carolina State UniversityRaleighUSA

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