Molecular Breeding

, Volume 27, Issue 2, pp 223–232

Analysis of alien introgression in coffee tree (Coffeaarabica L.)

  • Philippe Lashermes
  • Marie-Christine Combes
  • Caroline Ansaldi
  • Elijah Gichuru
  • Sandra Noir
Article

DOI: 10.1007/s11032-010-9424-2

Cite this article as:
Lashermes, P., Combes, M., Ansaldi, C. et al. Mol Breeding (2011) 27: 223. doi:10.1007/s11032-010-9424-2

Abstract

The transfer of desired traits from related wild diploid Coffea species into the cultivated allotetraploid C. arabica is essential in coffee breeding to develop pest/disease-resistant cultivars. The present work is an attempt to gain insights into alien introgression in C. arabica. An F2 population derived from a cross between T5296 and Et6 was analysed with simple sequence repeat (SSR; microsatellite) and amplified fragment length polymorphism (AFLP) molecular markers. The T5296 is a derivative of an interspecific hybrid introgressed by the diploid C. canephora species and Et6 is a wild Ethiopian accession of C. arabica. The origin of the revealed polymorphism was determined by comparisons using representative accessions from C. arabica and its two diploid parental species, C. eugenioides and C. canephora. The number and mode of inheritance of canephora-introgressed segments were investigated, as well as their sub-genome localisation and rate of recombination. The results suggested that the transfer of desirable genes into C. arabica from C. canephora is not limited by the ploidy level differences or the suppression of recombination between the different genomes.

Keywords

IntrogressionRecombinationInheritanceCoffeePolyploidyMolecular marker

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Philippe Lashermes
    • 1
  • Marie-Christine Combes
    • 1
  • Caroline Ansaldi
    • 1
  • Elijah Gichuru
    • 1
    • 2
  • Sandra Noir
    • 1
    • 3
  1. 1.Institut de Recherche pour le Développement (IRD)UMR RPB (CIRAD, IRD, UM2)Montpellier Cedex 5France
  2. 2.Coffee Research Foundation (CRF)RuiruKenya
  3. 3.School of Biological SciencesRoyal Holloway University of LondonEgham, SurreyUK