Article

Molecular Breeding

, Volume 27, Issue 2, pp 181-192

Genetic linkage map of melon (Cucumis melo L.) and localization of a major QTL for powdery mildew resistance

  • Fernando J. Yuste-LisbonaAffiliated withDepartamento de Mejora Vegetal, Estación Experimental ‘La Mayora’ (EELM), Consejo Superior de Investigaciones Científicas (CSIC)
  • , Carmen CapelAffiliated withDepartamento de Biología Aplicada (Genética), Edificio CITE II-B, Universidad de Almería
  • , Emilio SarriaAffiliated withDepartamento de Mejora Vegetal, Estación Experimental ‘La Mayora’ (EELM), Consejo Superior de Investigaciones Científicas (CSIC)
  • , Rocío TorreblancaAffiliated withDepartamento de Biología Aplicada (Genética), Edificio CITE II-B, Universidad de Almería
  • , María L. Gómez-GuillamónAffiliated withDepartamento de Mejora Vegetal, Estación Experimental ‘La Mayora’ (EELM), Consejo Superior de Investigaciones Científicas (CSIC)
  • , Juan CapelAffiliated withDepartamento de Biología Aplicada (Genética), Edificio CITE II-B, Universidad de Almería
  • , Rafael LozanoAffiliated withDepartamento de Biología Aplicada (Genética), Edificio CITE II-B, Universidad de Almería Email author 
  • , Ana I. López-SeséAffiliated withDepartamento de Mejora Vegetal, Estación Experimental ‘La Mayora’ (EELM), Consejo Superior de Investigaciones Científicas (CSIC)

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Abstract

Powdery mildew caused by Podosphaera xanthii has become a major problem in melon since it occurs all year round irrespective of the growing system. The TGR-1551 melon genotype was found to be resistant to several melon diseases, among them powdery mildew. However, the corresponding resistance genes have been never mapped. We constructed an integrated genetic linkage map using an F2 population derived from a cross between the multi-resistant genotype TGR-1551 and the susceptible Spanish cultivar ‘Bola de Oro’. The map spans 1,284.9 cM, with an average distance of 3.6 cM among markers, and consists of 354 loci (188 AFLP, 39 RAPD, 111 SSR, 14 SCAR/CAPS/dCAPS, and two phenotypic traits) distributed in 14 linkage groups. QTL analysis identified one major QTL (Pm-R) on LG V for resistance to races 1, 2, and 5 of powdery mildew. The PM4-CAPS marker is closely linked to the Pm-R QTL at a genetic distance of 1.9 cM, and the PM3-CAPS marker is located within the support interval of this QTL. These codominant markers, together with the map information reported here, could be used for melon breeding, and particularly for genotyping selection of resistance to powdery mildew in this vegetable crop species.

Keywords

Codominant DNA markers Disease resistance Podosphaera xanthii Quantitative trait loci