Molecular Breeding

, Volume 27, Issue 1, pp 77–92

Development of wild barley-derived DArT markers and their integration into a barley consensus map

Authors

  • B. P. Alsop
    • Department of Plant PathologyUniversity of Minnesota
  • A. Farre
    • Centre UdL-IRTAUniversidad de Lleida
  • P. Wenzl
    • Triticarte P/L and DArT P/L
  • J. M. Wang
    • Institute of Crop and Nuclear Technology UtilizationZhejiang Academy of Agricultural Sciences
  • M. X. Zhou
    • Tasmanian Institute of Agricultural ResearchUniversity of Tasmania
  • I. Romagosa
    • Centre UdL-IRTAUniversidad de Lleida
  • A. Kilian
    • Triticarte P/L and DArT P/L
    • Department of Plant PathologyUniversity of Minnesota
Article

DOI: 10.1007/s11032-010-9415-3

Cite this article as:
Alsop, B.P., Farre, A., Wenzl, P. et al. Mol Breeding (2011) 27: 77. doi:10.1007/s11032-010-9415-3

Abstract

Wild barley-specific genomic libraries were developed for the purpose of creating a ‘comprehensive’ genomic representation of the primary Hordeum genepool capable of more robust genotyping of barley. In order to enrich for wild barley-specific sequences in the DArT libraries, suppression subtraction hybridization (SSH) was performed using cultivated barley as the subtraction driver and wild barley as the tester. Four doubled-haploid populations were genotyped with the comprehensive barley DArT array, including two from wild × cultivated crosses (Damon/Harrington and Shechem/Harrington) and two from cultivated × cultivated crosses (Albacete/Barbarrouse and TX9425/Naso Nijo). Analysis of genotyping data revealed that the SSH process was somewhat ineffective at enriching for unique sequences in this application of DArT marker development. However, the addition of markers derived from wild barley proved to be an effective means for increasing the number of polymorphic markers obtainable from a single DArT assay. Genetic maps of the four component populations were developed and 607 newly developed DArT markers were integrated with a barley consensus map to create a new synthetic map of the barley genome containing 3542 markers. This significantly increased the resolution of the consensus map and improved the power of the map to provide a reference for profiling genetic diversity within the primary Hordeum genepool. The improvement in the genotyping capability of the comprehensive DArT genomic representation and the higher resolution of the synthetic map facilitates an even greater flexibility of DArT markers to be utilized as a fast, high-throughput platform for molecular marker-based barley breeding.

Keywords

Hordeum vulgare subsp. spontaneumSynthetic mapDiversity arrays technology

Supplementary material

11032_2010_9415_MOESM1_ESM.xls (5.5 mb)
Supplementary material 1 (XLS 5634 kb)
11032_2010_9415_MOESM2_ESM.tif (29.8 mb)
Supplementary material 2 (TIFF 30518 kb)
11032_2010_9415_MOESM3_ESM.xls (602 kb)
Supplementary material 3 (XLS 602 kb)

Copyright information

© Springer Science+Business Media B.V. 2010