Molecular Breeding

, Volume 26, Issue 4, pp 667–680

Haplotype diversity of stem rust resistance loci in uncharacterized wheat lines

  • Long-Xi Yu
  • Sixin Liu
  • James A. Anderson
  • Ravi P. Singh
  • Yue Jin
  • Jorge Dubcovsky
  • Gina Brown-Guidera
  • Sridhar Bhavani
  • Alexey Morgounov
  • Zhonghu He
  • Julio Huerta-Espino
  • Mark E. Sorrells
Article

DOI: 10.1007/s11032-010-9403-7

Cite this article as:
Yu, LX., Liu, S., Anderson, J.A. et al. Mol Breeding (2010) 26: 667. doi:10.1007/s11032-010-9403-7

Abstract

Stem rust is one of the most destructive diseases of wheat worldwide. The recent emergence of wheat stem rust race Ug99 (TTKS based on the North American stem rust race nomenclature system) and related strains threaten global wheat production because they overcome widely used genes that had been effective for many years. Host resistance is likely to be more durable when several stem rust resistance genes are pyramided in a single wheat variety; however, little is known about the resistance genotypes of widely used wheat germplasm. In this study, a diverse collection of wheat germplasm was haplotyped for stem rust resistance genes Sr2, Sr22, Sr24, Sr25, Sr26, Sr36, Sr40, and 1A.1R using linked microsatellite or simple sequence repeat (SSR) and sequence tagged site (STS) markers. Haplotype analysis indicated that 83 out of 115 current wheat breeding lines from the International Maize and Wheat Improvement Center (CIMMYT) likely carry Sr2. Among those, five out of 94 CIMMYT spring lines tested had both Sr2 and Sr25 haplotypes. Five out of 22 Agriculture Research Service (ARS) lines likely have Sr2 and a few have Sr24, Sr36, and 1A.1R. Two out of 43 Chinese accessions have Sr2. No line was found to have the Sr26 and Sr40 haplotypes in this panel of accessions. DArT genotyping was used to identify new markers associated with the major stem resistance genes. Four DArT markers were significantly associated with Sr2 and one with Sr25. Principal component analysis grouped wheat lines from similar origins. Almost all CIMMYT spring wheats were clustered together as a large group and separated from the winter wheats. The results provide useful information for stem rust resistance breeding and pyramiding.

Keywords

Stem rustSr geneHaplotypePyramidingGenetic relationshipMarker-assisted selection

Supplementary material

11032_2010_9403_MOESM1_ESM.doc (232 kb)
Supplementary material 1 (DOC 232 kb)

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Long-Xi Yu
    • 1
  • Sixin Liu
    • 2
  • James A. Anderson
    • 2
  • Ravi P. Singh
    • 3
  • Yue Jin
    • 4
  • Jorge Dubcovsky
    • 5
  • Gina Brown-Guidera
    • 6
  • Sridhar Bhavani
    • 3
  • Alexey Morgounov
    • 3
  • Zhonghu He
    • 3
    • 7
  • Julio Huerta-Espino
    • 8
  • Mark E. Sorrells
    • 1
  1. 1.Department of Plant Breeding and GeneticsCornell UniversityIthacaUSA
  2. 2.Department of Agronomy and Plant GeneticsUniversity of MinnesotaSt. PaulUSA
  3. 3.International Maize and Wheat Improvement Center (CIMMYT)Edo MexMexico
  4. 4.USDA-ARSCereal Disease LaboratorySt. PaulUSA
  5. 5.Department of Plant SciencesUniversity of CaliforniaDavisUSA
  6. 6.USDA-ARS Plant Science ResearchRaleighUSA
  7. 7.Chinese Academy of Agriculture ScienceBeijingChina
  8. 8.Campo Experimental Valle de México INIFAPChapingoMexico