Molecular Breeding

, Volume 25, Issue 1, pp 167–177

Molecular marker linked to a chromosome region regulating seed Zn accumulation in barley

Authors

    • Soil Science and Plant Nutrition, Faculty of Natural and Agricultural SciencesUniversity of Western Australia
    • Dryland Agricultural Research Institute (DARI)
  • Zed Rengel
    • Soil Science and Plant Nutrition, Faculty of Natural and Agricultural SciencesUniversity of Western Australia
  • Chengdao Li
    • Soil Science and Plant Nutrition, Faculty of Natural and Agricultural SciencesUniversity of Western Australia
    • Department of Agriculture & Food
  • Hua’an Yang
    • Department of Agriculture & Food
Article

DOI: 10.1007/s11032-009-9317-4

Cite this article as:
Sadeghzadeh, B., Rengel, Z., Li, C. et al. Mol Breeding (2010) 25: 167. doi:10.1007/s11032-009-9317-4

Abstract

Zinc deficiency is a critical nutritional problem in soils, restricting yield and nutritional quality of barley (Hordeum vulgare L.). Some genotypes (Zn-efficient) can produce greater yield and accumulate more Zn in seed under Zn deficiency than standard (Zn-inefficient) genotypes. However, there is little information regarding the genetics of Zn uptake/accumulation and location of genes conferring Zn efficiency in barley. Selection through molecular markers for seed Zn accumulation might be an efficient complementary breeding tool in barley. With the aim of developing molecular markers for increased accumulation of Zn in seed, a population of 150 DH lines derived from a cross between Clipper (low-Zn-accumulator) and Sahara 3771 (high-Zn-accumulator) was screened in the field and glasshouse for seed Zn concentration and content. One dominant DNA polymorphism was detected using the microsatellite-anchored fragment length polymorphism (MFLP) technique. The candidate MFLP marker was isolated from the MFLP gel, re-amplified by PCR, cloned, sequenced, and converted into simple sequence-specific and PCR-based marker. This marker, located on the short arm of chromosome 2H, might be useful for the improvement of barley nutritional quality and productivity programs in Zn-deficient environments. However, high seed Zn alone can not replace the need for Zn fertilization.

Keywords

MFLP Molecular marker Seed Zn accumulation Barley Zn efficiency

Copyright information

© Springer Science+Business Media B.V. 2009