Molecular Breeding

, Volume 23, Issue 1, pp 153–161

Development of a sequence-specific PCR marker linked to the gene “pauper” conferring low-alkaloids in white lupin (Lupinus albus L.) for marker assisted selection

  • Ruiming Lin
  • Daniel Renshaw
  • David Luckett
  • Jonathon Clements
  • Guijun Yan
  • Kedar Adhikari
  • Bevan Buirchell
  • Mark Sweetingham
  • Huaan Yang
Article

DOI: 10.1007/s11032-008-9222-2

Cite this article as:
Lin, R., Renshaw, D., Luckett, D. et al. Mol Breeding (2009) 23: 153. doi:10.1007/s11032-008-9222-2

Abstract

Seeds and plants of wild type Lupinus albus are bitter and contain high level of alkaloids. During domestication, at least three genes conferring low-alkaloid content were identified and incorporated into commercial varieties. Australian lupin breeders exclusively utilize one of these sweetness genes, “pauper”, in all varieties to prevent possible bitterness contamination via out-crossing. A cross was made between a sweet variety Kiev Mutant (containing pauper gene) and a bitter type landrace P27174, and the population was advanced into F8 recombinant inbred lines (RILs). Twenty-four plants representing sweetness and bitterness were subjected to DNA fingerprinting by the microsatellite-anchored fragment length polymorphism (MFLP) technique. A dominant polymorphism was discovered in an MFLP fingerprint. The MFLP marker was converted into a co-dominant, sequence-specific, simple PCR-based marker. Linkage analysis by the software program MapManager with marker score data and alkaloid phenotyping data from a segregating population containing 190 F8 RILs indicated that the marker is linked to the pauper gene at the genetic distance of 1.4 centiMorgans (cM). This marker, which is designated as “PauperM1”, is capable of distinguishing the pauper gene from the other two low-alkaloid genes exiguus and nutricius. Validation on germplasm from the Australian lupin breeding program showed that the banding pattern of the marker PauperM1 is consistent with the alkaloid genotyping on a wide range of domesticated varieties and breeding lines. The PauperM1 marker is now being implemented for marker assisted selection in the Australian albus lupin breeding program.

Keywords

Marker assisted selection (MAS)Molecular markerMFLPLupinus albus

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Ruiming Lin
    • 1
  • Daniel Renshaw
    • 2
  • David Luckett
    • 3
  • Jonathon Clements
    • 2
    • 4
  • Guijun Yan
    • 5
  • Kedar Adhikari
    • 2
  • Bevan Buirchell
    • 2
  • Mark Sweetingham
    • 2
  • Huaan Yang
    • 2
  1. 1.Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingPeople’s Republic of China
  2. 2.Department of Agriculture and Food Western AustraliaSouth PerthAustralia
  3. 3.EH Graham Centre for Agricultural InnovationNSW Department of Primary Industries, Agricultural InstituteWagga WaggaAustralia
  4. 4.The Centre for Legumes in Mediterranean AgricultureThe University of Western AustraliaCrawleyAustralia
  5. 5.School of Plant Biology, Faculty of Natural and Agricultural SciencesThe University of Western AustraliaCrawleyAustralia