Molecular Breeding

, Volume 21, Issue 4, pp 431–438

Expression and function of two dehydrins under environmental stresses in Brassica juncea L.

  • Jin Xu
  • Yuxiu Zhang
  • Ziqiu Guan
  • Wei Wei
  • Lu Han
  • Tuanyao Chai
Article

DOI: 10.1007/s11032-007-9143-5

Cite this article as:
Xu, J., Zhang, Y., Guan, Z. et al. Mol Breeding (2008) 21: 431. doi:10.1007/s11032-007-9143-5

Abstract

Dehydrins are capable of conferring abiotic stress tolerance in plants. Few dehydrins have been characterized at the molecular and transgenic level in Brassica juncea. In the present study, two SK2-type B. juncea dehydrin genes, BjDHN2 and BjDHN3, showed improved stress tolerance to salt and freezing stress in transgenic yeast. Semiquantitative reverse transcription polymerase chain reaction (RT-PCR) revealed that BjDHN2 and BjDHN3 were up-regulated by low temperature, drought, and salt stress, as well as exogenous abscisic acid (ABA) in B. juncea. Transgenic studies also showed that BjDHN2 and BjDHN3 increased tolerance to cold and salt stress in tobacco. These results indicate that the BjDHN2 and BjDHN3 genes exert a protective role on membranes and play a role in abiotic stresses.

Keywords

Brassica junceaDehydrinAbiotic stressYeastTransgenic tobacco

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Jin Xu
    • 1
  • Yuxiu Zhang
    • 2
  • Ziqiu Guan
    • 1
  • Wei Wei
    • 1
  • Lu Han
    • 1
  • Tuanyao Chai
    • 1
  1. 1.Department of BiologyGraduate University of Chinese Academy of SciencesBeijingP.R. China
  2. 2.Department of Biological Engineering, School of Chemical and Environmental EngineeringChina University of Mining and Technology (Beijing)BeijingP.R. China