Molecular Breeding

, Volume 20, Issue 3, pp 233–248

Increase of glycinebetaine synthesis improves drought tolerance in cotton

Authors

  • Sulian Lv
    • School of Life SciencesShandong University
  • Aifang Yang
    • School of Life SciencesShandong University
  • Kewei Zhang
    • School of Life SciencesShandong University
  • Lei Wang
    • School of Life SciencesShandong University
    • School of Life SciencesShandong University
Article

DOI: 10.1007/s11032-007-9086-x

Cite this article as:
Lv, S., Yang, A., Zhang, K. et al. Mol Breeding (2007) 20: 233. doi:10.1007/s11032-007-9086-x

Abstract

The tolerance to drought stress of the homozygous transgenic cotton (Gossypium hirsutum L.) plants with enhanced glycinebetaine (GB) accumulation was investigated at three development stages. Among the five transgenic lines investigated, lines 1, 3, 4, and 5 accumulated significantly higher levels of GB than the wild-type (WT) plants either before or after drought stress, and the transgenic plants were more tolerant to drought stress than the wild-type counterparts from young seedlings to flowering plants. Under drought stress conditions, transgenic lines 1, 3, 4, and 5 had higher relative water content, increased photosynthesis, better osmotic adjustment (OA), a lower percentage of ion leakage, and less lipid membrane peroxidation than WT plants. The GB levels in transgenic plants were positively correlated with drought tolerance under water stress. The results suggested that GB may not only protect the integrity of the cell membrane from drought stress damage, but also be involved in OA in transgenic cotton plants. Most importantly, the seedcotton yield of transgenic line 4 was significantly greater than that of WT plants after drought stress, which is of great value in cotton production.

Keywords

Drought stressGlycinebetaineTransgenic cotton

Abbreviations

BADH

Betaine aldehyde dehydrogenase

CDH

Choline dehydrogenase

CMO

Choline monooxygenase

GB

Glycinebetaine

MDA

Malondialdehyde

OA

Osmotic adjustment

PVP

Polyvinylpyrrolidone

ROS

Reactive oxygen species

RWC

Relative water content

SOD

Superoxide dismutase

WT

Wild-type

Copyright information

© Springer Science+Business Media B.V. 2007