Microbiology

, Volume 74, Issue 1, pp 54–59

The synthesis of hydroxybutyrate and hydroxyvalerate copolymers by the bacterium Ralstonia eutropha

  • T. G. Volova
  • G. S. Kalacheva
Experimental Articles

DOI: 10.1007/s11021-005-0028-5

Cite this article as:
Volova, T.G. & Kalacheva, G.S. Microbiology (2005) 74: 54. doi:10.1007/s11021-005-0028-5

Abstract

The paper deals with the study of the synthesis of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) copolymers by the bacterium Ralstonia eutropha B-5786 grown under different carbon nutrition conditions (growth on carbon dioxide, fructose, and CO2-valerate and fructose-valerate mixtures). The parameters to be analyzed included the yield of biomass; the yield, synthesis rate, and composition of copolymers; the activity of the key enzymes of polyhydroxyalkanoate (PHA) synthesis β-ketothiolase, acetoacetyl-CoA reductase, and PHA synthase); the maximum tolerable concentration of valerate to the bacterium; and the conditions that govern the incorporation of hydroxyvalerate into copolymers. This allowed the relationship between cultivation conditions and the proportion of monomers in the copolymers to be deduced. We were able to synthesize a range of 3HB/3HV copolymers and found that the thermal characteristics and the degree of crystallinity of such copolymers depend on the molar fraction of 3HV.

Key words

poly(hydroxybutyrate-co-hydroxyvalerate)controlled synthesisβ-ketothiolaseRalstonia eutropha

Copyright information

© MAIK “Nauka/Interperiodica” 2005

Authors and Affiliations

  • T. G. Volova
    • 1
  • G. S. Kalacheva
    • 1
  1. 1.Institute of Biophysics, Siberian DivisionRussian Academy of SciencesKrasnoyarskRussia