Metabolic Brain Disease

, Volume 28, Issue 3, pp 439–446

Modulation of crucial adenosinetriphosphatase activities due to U-74389G administration in a porcine model of intracerebral hemorrhage

  • Alexios Bimpis
  • Apostolos Papalois
  • Stylianos Tsakiris
  • Konstantinos Kalafatakis
  • Apostolos Zarros
  • Vasiliki Gkanti
  • Nikolina Skandali
  • Hussam Al-Humadi
  • Constantinos Kouzelis
  • Charis Liapi
Original Paper

DOI: 10.1007/s11011-013-9380-8

Cite this article as:
Bimpis, A., Papalois, A., Tsakiris, S. et al. Metab Brain Dis (2013) 28: 439. doi:10.1007/s11011-013-9380-8

Abstract

Spontaneous intracerebral hemorrhage (ICH) represents a partially-understood cerebrovascular disease of high incidence, morbidity and mortality. We, herein, report the findings of our study concerning the role of two important adenosinetriphosphatases (ATPases) in a porcine model of spontaneous ICH that we have recently developed (by following recent references as well as previously-established models and techniques), with a focus on the first 4 and 24 h following the lesion’s induction, in combination with a study of the effectiveness of the lazaroid antioxidant U-74389G administration. Our study demonstrates that the examined ICH model does not cause a decrease in Na+,K+-ATPase activity (the levels of which are responsible for a very large part of neuronal energy expenditure) in the perihematomal basal ganglia territory, nor a change in the activity of Mg2+-ATPase. This is the first report focusing on these crucial ATPases in the experimental setting of ICH and differs from the majority of the findings concerning the behavior of these (crucial for central nervous system cell survival) enzymes under stroke-related ischemic conditions. The administration of U-74389G (an established antioxidant) in this ICH model revealed an injury specific type of behavior, that could be considered as neuroprotective provided that one considers that Na+,K+- and Mg2+-ATPase inhibition might in this case diminish the local ATP consumption.

Keywords

Intracerebral hemorrhagePorcine modelLazaroidU-74389GAdenosinetriphosphatasesNa+, K+-ATPaseMg2+-ATPaseActivityATP

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Alexios Bimpis
    • 1
  • Apostolos Papalois
    • 2
  • Stylianos Tsakiris
    • 3
  • Konstantinos Kalafatakis
    • 1
    • 3
  • Apostolos Zarros
    • 1
    • 3
  • Vasiliki Gkanti
    • 1
    • 3
  • Nikolina Skandali
    • 1
    • 3
  • Hussam Al-Humadi
    • 1
  • Constantinos Kouzelis
    • 4
  • Charis Liapi
    • 1
  1. 1.Department of Pharmacology, Medical SchoolNational and Kapodistrian University of AthensAthensGreece
  2. 2.Experimental - Research Center ELPENAthensGreece
  3. 3.Department of Physiology, Medical SchoolNational and Kapodistrian University of AthensAthensGreece
  4. 4.Department of NeurosurgeryThriassio General HospitalAthensGreece