Metabolic Brain Disease

, Volume 28, Issue 3, pp 429–438

Methylglyoxal-induced cytotoxicity in neonatal rat brain: a role for oxidative stress and MAP kinases

  • Luana Heimfarth
  • Samanta Oliveira Loureiro
  • Paula Pierozan
  • Bárbara Ortiz de Lima
  • Karina Pires Reis
  • Elisandra Barbosa Torres
  • Regina Pessoa-Pureur
Original Paper

DOI: 10.1007/s11011-013-9379-1

Cite this article as:
Heimfarth, L., Loureiro, S.O., Pierozan, P. et al. Metab Brain Dis (2013) 28: 429. doi:10.1007/s11011-013-9379-1

Abstract

Carbonyl compounds such as methylglyoxal (MGO) seem to play an important role in complications resulting from diabetes mellitus, in aging and neurodegenerative disorders. In this study, we are showing, that MGO is able to suppress cell viability and induce apoptosis in the cerebral cortex and hippocampus of neonatal rats ex-vivo. These effects are partially related with ROS production, evaluated by DCFH-DA assay. Coincubation of MGO and reduced glutathione (GSH) or Trolox (vitamin E) totally prevented ROS production but only partially prevented the MGO-induced decreased cell viability in the two brain structures, as evaluated by the MTT assay. Otherwise, L-NAME, a nitric oxide (NO) inhibitor, partially prevented ROS production in the two structures but partially prevented cytotoxicity in the hippocampus. Pharmacological inhibition of Erk, has totally attenuated MGO-induced ROS production and cytotoxicity, suggesting that MEK/Erk pathway could be upstream of ROS generation and cell survival. Otherwise, p38MAPK and JNK failed to prevent ROS generation but induced decreased cell survival consistent with ROS-independent mechanisms. We can propose that Erk, p38MAPK and JNK are involved in the cytotoxicity induced by MGO through different signaling pathways. While Erk could be an upstream effector of ROS generation, p38MAPK and JNK seem to be associated with ROS-independent cytotoxicity in neonatal rat brain. The cytotoxic damage progressed to apoptotic cell death at MGO concentration higher than those described for adult brain, suggesting that the neonatal brain is resistant to MGO-induced cell death. The consequences of MGO-induced brain damage early in life, remains to be clarified. However, it is feasible that high MGO levels during cortical and hippocampal development could be, at least in part, responsible for the impairment of cognitive functions in adulthood.

Keywords

MethylglyoxalOxidative stressMAP kinasesNeonatal rat brainCerebral cortexHippocampus

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Luana Heimfarth
    • 1
  • Samanta Oliveira Loureiro
    • 1
  • Paula Pierozan
    • 1
  • Bárbara Ortiz de Lima
    • 1
  • Karina Pires Reis
    • 1
  • Elisandra Barbosa Torres
    • 1
  • Regina Pessoa-Pureur
    • 1
  1. 1.Departamento de Bioquímica, Instituto de Ciências Básicas da SaúdeUniversidade Federal do Rio Grande do SulPorto AlegreBrazil