Metabolic Brain Disease

, 24:469

Protective effect of antioxidants on cerebrum oxidative damage caused by arginine on pyruvate kinase activity

  • Débora Delwing
  • Daniela Delwing de Lima
  • Bianca Scolaro
  • Gabriela G. Kuss
  • José G. P. Cruz
  • Angela T. S. Wyse
Original Paper

DOI: 10.1007/s11011-009-9152-7

Cite this article as:
Delwing, D., Delwing de Lima, D., Scolaro, B. et al. Metab Brain Dis (2009) 24: 469. doi:10.1007/s11011-009-9152-7
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Abstract

We have demonstrated that acute arginine administration decreases antioxidant defenses and compromises enzymes of respiratory chain in rat brain. In this study we evaluated in vivo and in vitro effect of arginine on pyruvate kinase activity, as well as its effect on an important parameter of oxidative stress namely thiobarbituric acid-reactive substances (TBA-RS) in cerebrum of rats. We also tested the influence of antioxidants, namely α -tocopherol plus ascorbic acid on the effects elicited by arginine in order to investigate the possible participation of free radicals on the effects of arginine on these parameters. Results showed that arginine acute administration inhibited pyruvate kinase activity in cerebrum of rats, as well as increased TBA-RS. By the other hand, arginine added to the incubation medium, in vitro studies, did not alter these parameters in rat cerebrum. In addition, pretreatment with antioxidants prevented the reduction of pyruvate kinase activity and the increase of TBA-RS caused by arginine. The data indicate that acute administration of arginine induces lipid peroxidation in rat cerebrum and that the inhibition of pyruvate kinase activity caused by this amino acid was probably mediated by free radicals since antioxidants prevented such effect. It is presumed that these results might be associated, at least in part, with the neuronal dysfunction of patients affected by hyperargininemia. Finally, we suggest that the administration of antioxidants should be considered as an adjuvant therapy to specific diets in hyperargininemia.

Keywords

ArgininePyruvate kinaseAntioxidantsCerebrumLipid peroxidation

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Débora Delwing
    • 1
  • Daniela Delwing de Lima
    • 2
  • Bianca Scolaro
    • 1
  • Gabriela G. Kuss
    • 1
  • José G. P. Cruz
    • 1
  • Angela T. S. Wyse
    • 3
  1. 1.Departamento de Ciências Naturais, Centro de Ciências Exatas e Naturais, Universidade Regional de BlumenauBlumenauBrasil
  2. 2.Área de Ciências da Saúde, Universidade Comunitária da Região de ChapecóChapecóBrasil
  3. 3.Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do SulPorto AlegreBrasil